MicroRNA targets the ecdysone signaling pathway E75 gene to control larval-pupal development in Bactrocera dorsalis  

作　　者：Wei Peng Wei-Wei Zheng Kaleem Tariq Shu-Ning Yu Hong-Yu Zhang 

机构地区：[1]Key laboratory of Horticultural Plant Biology (MOE) and Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China [2]Department of Agriculture, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, Pakistan

出　　处：《Insect Science》2019年第2期229-239,共11页昆虫科学（英文版）

基　　金：the earmarked fund for the China Agricultural Research System (No. CARS-26);International Atomic Energy Agency's Coordinated Research Project (No. D42016);the National Natural Science Foundation of China (No. 31572008);Fundamental Research Funds for the Central Universities (No. 2014PY005 and 2662015PY129).

摘　　要：MicroRNAs (miRNAs) regulate various biological processes during insect developme nt;however, their role in larval-pupal development in oriental fruit fly, Bactrocera dorsalis (Hendel) remains unknown. In the current study, we address the biological function of a conserved miRNA, Bdo-Let-7 in the regulation of BdE75 gene, which belongs to the ecdysone signaling pathway and participates in the larval-pupal development in B. dorsalis. Using dual luciferase reporter assay in HEK293T cells we show that Bdo-Let-7 miRNA interacts with the 3' untranslated region of BdE75 gene and suppresses its expression. The Bdo-Let?7 and BdE75 are also co-expressed in the larval-pupal stages and in different tissues of B. dorsalis .In in vivo experiments, the injectio n of Bdo-Let-7 agomir and antagomir in third instar larvae down- and up-regulated the expression of BdE75、 respectively. The 20-hydroxyecdysone (20E) injection assay shows that 20E up-regulated the expression of Bdo-Let-7 on the 5th day of the larvae. Moreover, abnormal pupation and eclosion were observed after larval Bdo-Let-7 antagomir injection. Based on these results, we show that Bdo-Let-7 regulates the ecdysone signaling pathway through the exact dose of BdE75 gene, and is indispensable for normal larval-pupal development in B. dorsalis.

关 键 词：BACTROCERA dorsalis BdE75 Bdo-Let-7 ECDYSONE signaling pathway 

分 类 号：Q[生物学]