BSL-3实验室医疗废弃物专用包装袋的封口方式及层数对高压灭菌效果的影响  

Impact of sealing method and layer number of autoclave bag on autoclave sterilization effect in the BSL-3 laboratory

作　　者：甄维[1] 蔡琨[1] 史海月 张靖[1] 韩卫芳[1] 周为民[1] 武桂珍[1] Zhen Wei;Cai Kun;Shi Haiyue;Zhang Jing;Han Weifang;Zhou Weimin;Wu Guizhen(Biosafety levels 3 laboratory,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所BSL-3实验室,北京102206

出　　处：《中华实验和临床病毒学杂志》2019年第1期30-33,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的对感染性废物的包装给出一个合理的包装层数和封口方式,在指定的高压灭菌程序下,达到更好的高压灭菌效果。方法在指定的高压程序P4、P9下,针对医疗废弃物专用包装袋采用不同的包装层数和封口方式(包装层数分别为单层、双层、三层;封口方式分别是轻微折叠、高压胶带、尼龙扎带封口);通过用生物指示剂对内部感染性废物的上部、中部、底部进行检测,评价不同组合方式下的高压灭菌效果。结果1)在P4高压程序下,三层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、下部的生物指示剂的阴性率分别为上部是100%、0%、0%,中部是45%、0%、0%,底部都是0%。二层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、底部的生物指示剂的阴性率分别为上部是100%、85%、0%,中部是67%、20%、0%,底部是10%、0%、0%。一层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、底部的生物指示剂的阴性率分别为上部是100%、100%、0%,中部是70%、45%、0%,底部是12.5%、0%、0%。(2)在P9高压程序下,三层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、底部的生物指示剂的阴性率分别为上部均是100%,中部是100%、100%、70%,底部是100%、100%、30%。二层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、底部的生物指示剂的阴性率分别为上部均是100%,中部均是100%,底部是100%、100%、60%。一层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、底部的生物指示Objective To evaluate,the method of different packing layer and sealing mode for the packaging of the infectious wastes in order to achieve better autoclave sterilization effect.Methods In the autoclave cycles P4 and P9,different packing layer (single,double and triple) and sealing mode (slightly folded,autoclave tape,nylon tape closure) were applied to the internal infectious waste sterilization,biological indicators were used to detect the effect of autoclave sterilization of the upper,middle and lower layers,respectively.Results 1) In the autoclave cycle P4,the triple autoclave bags packaging sealing method was based on the slight folding,autoclave tape,and nylon taple,the biological indicator negative rate of the internal infectious waste in the upper layer,the middle layer and the lower layer were respectively in the upper layer 100%,0%,0%,in the middle layer 67%,20%,0%,and in the lower layer was 0%.The double autoclave bags packaging sealing method was based on the slight folding,autoclave tape,and nylon taple,the biological indicator negative rate of the internal infectious waste in the upper layer,the middle layer and the lower layer were respectively the in the upper layer 100%,85%,0%,the middle layer 45%,0%,0%,and the lower layer 10%,0%,0%.the single autoclave bag packaging sealing method wa based on the slight folding,autoclave tape,and nylon taple,the biological indicator negative rate of the internal infectious waste in the upper,middle and lower layers were respectively the upper layer 100%,100%,0%,the middle layer 70%,45%,0%,and the lower layer 12.5%,0%,0%.(2) In the autoclave cycle P9,the triple autoclave bags packaging sealing method was based on the slight folding,autoclave tape,and nylon taple,the biological indicator negative rate of the internal infectious waste in the upper layer,middle and lower layers were respectively the upper layer all 100%,the middle layer 100%,100%,70%,and the lower layer 100%,100%,30%.The double autoclave bags packaging sealing method was based on the slight folding,

关 键 词：BSL-3实验室 高压灭菌 医疗废弃物专用包装袋 

分 类 号：R197.21[医药卫生—卫生事业管理]