青海省2017年脊髓灰质炎病毒学监测结果分析  

Virological monitoring and analysis of polioviruses in Qinghai 2017

作　　者：范丽霞[1] 巴卓玛[1] 赵生仓 李崇亥 祝双利[2] Fan Lixia;Ba Zhuoma;Zhao Shengcang;Li Chonghai;Zhu Shuangli(Qinghai Center for Disease Control and Prevention,Xining 810007,China;National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China)

机构地区：[1]青海省疾病预防控制中心卫生检验检测中心,西宁810007 [2]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《中华实验和临床病毒学杂志》2019年第1期58-60,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的评价青海省2017年脊髓灰质炎(脊灰)病毒学监测情况。方法按照世界卫生组织(world health organization,WHO)第4版《脊灰实验室手册操作规程进行病毒分离,分离到的L20B阳性分离物做型内鉴定(intratypic differentiation,ITD),由中国疾病预防控制中心(chinese center for disease control and prevention,CDC)病毒病预防控制所国家脊灰实验室对脊灰病毒(poliovirus,PV)衣壳蛋白(capsid protein,VP1)编码区进行核苷酸序列测定和分析,对青海省2017年报告的急性弛缓性麻痹(acute flaccid paralysis,AFP)病例及健康儿童粪便标本进行脊灰病毒学监测和结果分析。结果2017年青海CDC脊灰实验室共收到AFP病例及健康儿童粪便标本211份,分离到PV2株,分离率为0.95%;非脊灰肠道病毒(non-polio enterovirus,NPEV)25株,分离率为11.85%;两株PV均为疫苗相关株。结论2017年青海CDC脊灰实验室在AFP病例及健康儿童粪便标本中未发现脊灰野病毒和疫苗衍生脊灰病毒,青海省继续维持无脊灰状态。Objective To evaluate 2017 poliovirus surveillance in Qinghai Province.Methods According to the World Health Organization (WHO) 4 th edition of the polio laboratory manual procedure for virus isolation,the isolated L20B positive strain was identified as intratypic differentiation (ITD) by the China Center for Disease Control and Prevention,CDC).The National Polio Laboratory performed the nucleotide sequence determination of the capsid protein VP1 coding region of poliovirus (PV) and analyzed the poliovirus surveillance and the result of analysis of the cases with acute flaccid paralysis (AFP) reported in Qinghai Province in 2017 and stool samples of healthy children.Results In 2017,Qinghai CDC Polio Laboratory received specimens of 211 AFP cases and healthy stool samples.PV2 strains were isolated with a separation rate of 0.95%.Non-polio enterovirus (NPEV) strains were isolated from 25 strains with the isolation rate of 11.85%.Two PVs were used for ITD.All of them were vaccine-associated strains.Conclusions In 2017,the Qinghai CDC Polio Laboratory did not find any poliovirus and vaccine- derived poliovirus in the AFP cases and stool samples from healthy persons,maintaining the polio-free status.

关 键 词：急性弛缓性麻痹 脊髓灰质炎病毒 病毒学监测 

分 类 号：R512.4[医药卫生—内科学] R181.3[医药卫生—临床医学]