慢性乙型肝炎peg干扰素疗效与TP区和RT区核酸研究  

Study on the association between the efficacy of peg-IFN and the complexity of TP and RT in chronic hepatitis B

作　　者：齐天林 王星月 冉崇平 曹卫华 张璐 路遥 郝红晓 吴淑玲 刘如玉 常敏 胡蕾苹 李明慧 谢尧 Qi Tianlin;Wang Xingyue;Ran Chongping;Cao Weihua;Zhang Lu;Lu Yao;Hao Hongxiao;Wu Shuling;Liu Ruyu;Chang Min;Hu Leiping;Li Minghui;Xie Yao(Dept.of Hepatology Division 2,Liver Diseases Center,Beijing Ditan Hospital,Capital Medical Universityt Beijing 100015,China)

机构地区：[1]首都医科大学附属北京地坛医院肝病中心肝病二科,北京100015

出　　处：《中华实验和临床病毒学杂志》2019年第1期74-78,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：首都临床特色应用研究专项资助课题(Z151100004015122);北京市医院管理局临床医学发展专项经费资助(XMLX201706);国家科技重大专项(2017ZX10201201-001-006);北京市科技计划子课题(D161100002716002);北京市医院管理局消化内科学科协同发展中心(中文简称:消化协同中心)资助课题(XXZ0302)。

摘　　要：目的探讨慢乙肝聚乙二醇干扰素治疗疗效与TP区和RT区复杂程度的相关性。方法入组HBeAg阳性,HBV DNA阳性的慢乙肝患者,给予聚乙二醇干扰素180μg每周1次皮下注射治疗,收集基线患者血清和患者基线与治疗12周临床信息。对基线HBV DNA的TP区和RT区片段进行扩增、建库、高通量测序,计算香农商和平均遗传距离。结果对108例患者进行logistic回归分析。RT区片段马氏距离和TP区片段的香农商有利于HBVDNA应答,ALT有利于HBeAg应答,HBsAg不利于HBsAg应答。结论基线TP区和RT区核酸可能与慢乙肝聚乙二醇干扰素治疗疗效有关。Objective To explore the association between the efficacy of peg-IFN and the complexity of TP and RT regions of hepatitis B virus (HBV) in chronic hepatitis B.Methods Patients with HBeAg positive,HBV DNA positive chronic hepatitis B were given peg-interferon 180 jxg once a week for subcutaneous injection,and baseline information was collected from baseline and after 12 weeks' treatment.The baseline HBV DNA TP and RT fragments were amplified,database,high-throughput sequencing,and the average genetic distance calculation.Results Data of 108 patients were analyzed by logistic regression.RT area fragment Markov distance and TP area fragment Shannon quotient for HBV DNA response were calculated.ALT level is good for HBeAg response.HBsAg level is bad for HBsAg response.Conclusions The complexity of the baseline TP and RT regions may be associated with the efficacy of peg-interferon therapy for CHB.

关 键 词：TP区 RT区 聚乙二醇干扰素 乙型肝炎病毒 

分 类 号：R512.62[医药卫生—内科学]