MALDI-TOF-MS在HBV相关肝癌蛋白质组学中的应用价值  

Application value of MALDI-TOF-MS in proteomics of hepatitis B virus-related liver cancer

作　　者：孙菲[1] 王刚[1] 张金仿[1] 苏建荣[1] Sun Fei;Wang Gang;Zhang Jinfang;Su Jianrong(Clinical Laboratory,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院检验科,北京100050

出　　处：《中华实验和临床病毒学杂志》2019年第1期95-98,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的探究基质辅助激光解吸电离飞行时间质谱技术(matrix assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF-MS)在HBV相关肝癌蛋白质组学中的应用价值。方法选取2018年1月—2018年6月在我院就诊的HBV相关肝癌患者30例,健康自愿者25例,弱阳离子磁珠捕获技术血清蛋白,运用MALDI-TOF-MS技术以及相关软件进行蛋白质分析,检测HBV相关肝癌的血清标志蛋白质,建立肝癌诊断模型,进行盲法验证。结果共筛选出81个蛋白质峰,而具有统计学差异的有27个(P<0.05),其中17个差异蛋白呈现高表达上调,10个差异蛋白呈现低表达下调。经BPS5.0软件筛选出3个差异蛋白峰(质荷比M/Z为9179.55、7789.00、4097.00)建模,后经盲法验证,其敏感性90.91%,特异性为77.78%。结论MALDI-TOF-MS技术为HBV相关肝癌的诊断和治疗提供了重要的依据。Objective To explore the application value of matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF-MS) inproteomics of HBV-related liver cancer.Methods Thirty patients with HBV-related liver cancer,and 25 healthy volunteers were selected from January2018 to June 2018 in our hospital.The serum proteins of these participantswere captured by weak cation exchange (WCX) beads.MALDI-TOF-MS and related software were used to analyze the proteins,detect the marker of serum proteins in HBV-related liver cancer,establish the liver cancer diagnosis model and perform blind test.Results Totally 81 protein peaks were screened out,and 27 protein peaks had significant difference (P<0.05),of which 17 were up-regulated,10 were down-regulated.Three differential protein peaks (M/Z 9179.55,7789.00,4097.00) were screened by BPS5.0 software to establish the model.Further blind test showed that the sensitivityand specificity of the protein peaks were 90.91 % and 77.78%,respectively.Conclusions MALDI-TOF-MS provided an important basis for the diagnosis and treatment of HBV-related liver cancer.

关 键 词：MALDI-TOF-MS 肝炎病毒 乙型 肝癌 蛋白质组学 

分 类 号：R512.62[医药卫生—内科学] R735.7[医药卫生—临床医学]