ob/ob小鼠平滑肌组织中富含半胱氨酸的酸性分泌蛋白的表达及临床意义  

Expression of SPARC in smooth muscle tissue of ob/ob mice and its clinical significance

作　　者：杨筱瑶 王亦薇[1] Yang Xiaoyao;Wang Yiwei(Department of Science and Education,Heilongjiang Provincial Hospital,Harbin Heilongjiang 150036,China)

机构地区：[1]黑龙江省医院科教部,哈尔滨150036

出　　处：《中国基层医药》2019年第4期385-387,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：黑龙江省卫生和计划生育委员会项目(2016-506);黑龙江省哈尔滨市科技局项目(2016RAXYJ091).

摘　　要：目的观察ob/ob小鼠(C57BL/6J-Lepob/J)平滑肌组织中富含半胱氨酸的酸性分泌蛋白(SPARC)的表达情况。方法研究时间:2017年2-6月,选择10周龄ob/ob小鼠(ob/ob组)及其同窝野生对照型小鼠(对照组)各6只,取其肠系膜动脉平滑肌组织,qRT-PCR方法检测平滑肌组织中SPARC基因表达,Western blot方法测定SPARC的目的蛋白表达。结果ob/ob组小鼠平滑肌组织中SPARC的mRNA(1.73±0.65),蛋白表达(1.45±0.43),明显高于对照组小鼠[(1.00±0.31),(1.00±0.33),t=8.437、5.533,均P<0.05]。结论SPARC在ob/ob小鼠平滑肌组织中呈现高表达,可能与糖尿病合并动脉粥样硬化有密切关系。Objective To investigate the secreted protein acidic and rich in cysteine(SPARC)expression in the smooth muscle tissue of ob/ob mice.MethodsStudy time:February 2017 to June 2017.Six ob/ob mice and 6 littermates aged 10 weeks were selected for the trial.The mesenteric artery smooth muscle tissues were collected.qRT-PCR was used to detect SPARC mRNA expression in smooth muscle tissue.The protein expression of SPARC was assayed by Western blot.ResultsThe SPARC mRNA[(1.73±0.65)]and protein[(1.73±0.65)]in smooth muscle tissue of ob/ob mice presented higher expression compared with those in the littermates[(1.00±0.31),(1.00±0.33),t=8.437,5.533,all P<0.05].ConclusionThe higher expression of SPARC in smooth muscles tissue of ob/ob mice may be involved in diabetes combined with atherosclerosis.

关 键 词：富含半胱氨酸的酸性分泌蛋白 小鼠 动脉平滑肌组织 糖尿病 肠系膜 表达 动脉粥样硬化 

分 类 号：R587.2[医药卫生—内分泌] R54[医药卫生—内科学]