姜黄素对模拟沙漠干热环境中暑大鼠外周血CD11b和CD19表达的影响  

Effect of curcumin on expressions of CD11b and CD19 in peripheral blood of heat stroke rats in a simulation dry-heat environment

作　　者：姜婧 刘江伟[1] 李佳佳[1] 陶林 王铮 杨柳 是文辉[1] 马娜[1] Jiang Jing;Liu Jiangwei;Li Jiajia;Tao Lin;Wang Zheng;Yang Liu;Shi Wenhui;Ma Na(Key Laboratory of Special Environmental Medicine, General Hospital of Xinjiang Military Command, Urumqi 830000, Xinjiang Uygur Autonomous Region, China)

机构地区：[1]新疆军区总医院新疆特殊环境医学重点实验室,新疆维吾尔自治区乌鲁木齐830000

出　　处：《中华危重病急救医学》2019年第2期221-224,共4页Chinese Critical Care Medicine

基　　金：新疆维吾尔自治区自然科学基金青年项目(2016D01C400).

摘　　要：目的探讨不同剂量姜黄素对模拟沙漠干热环境中暑大鼠外周血免疫因子CD11b和CD19水平的影响。方法选取SPF级健康雄性SD大鼠160只,按随机数字表法分为生理盐水(NS)对照组(给予NS)、溶剂对照组〔给予羧甲基纤维素钠(CMCNa)〕以及姜黄素低、中、高剂量预处理组(给予0.05、0.10、0.20 mg/g姜黄素+ 0.5% CMCNa溶液),每组32只,均按10 mL·kg-1·d-1灌胃,连续给药7 d。第8天将大鼠置于环境温度(41.0±0.5)℃、相对湿度(10±1)%的西北特殊环境人工实验舱中,于放置0(常温放置)、50、100和150 min时,用流式细胞仪检测各组大鼠外周血CD19和CD11b水平。结果随着在模拟干热环境中放置时间的延长,各组大鼠外周血CD11b水平逐渐升高,于150 min时达峰值,NS对照组、溶剂对照组以及姜黄素低、中、高剂量预处理组分别为0.346±0.013、0.342±0.013、0.342±0.012、0.325±0.012、0.281±0.012;而各组CD19水平先升高后降低,于100 min时达峰值,NS对照组、溶剂对照组以及姜黄素低、中、高剂量预处理组分别为0.586±0.010、0.601±0.014、0.684±0.009、0.613±0.012、0.604±0.006。姜黄素中、高剂量预处理组于干热环境下50 min和100 min时CD11b水平明显低于NS对照组和溶剂对照组(50 min:0.237±0.011、0.188±0.006比0.283±0.009、0.289±0.012,100 min:0.260±0.010、0.248±0.008比0.293±0.008、0.290±0.007,均P<0.05),150 min时姜黄素高剂量预处理组CD11b水平均明显低于NS对照组、溶剂对照组以及姜黄素低剂量组(0.281±0.012比0.346±0.013、0.342±0.013、0.342±0.012,均P<0.05)。干热环境下50 min时,姜黄素低、中、高剂量预处理组CD19水平均明显高于NS对照组和溶剂对照组(0.394±0.001、0.436±0.009、0.553±0.011比0.205±0.005、0.197±0.003,均P<0.05),100 min时仅姜黄素低剂量预处理组CD19水平明显高于NS对照组和溶剂对照组(0.684±0.009比0.586±0.010、0.601±0.014,均P<0.05),其余各剂量预处理组CD19水平与NS�Objective To investigate the effects of different doses of curcumin on the levels of immune factors CD11b and CD19 in peripheral blood of heat stroke rats in a simulation dry-heat environment. Methods 160 SPF healthy male Sprague-Dawley (SD) rats were selected and divided into different groups according to random number table method: normal saline (NS) control group (given NS), solvent control group [given sodium carboxymethylcellulose (CMCNa)], and curcumin low, medium and high dose pretreatment group (given 0.05, 0.10, 0.20 mg/g of curcumin+0.5% CMCNa solution). There were 32 rats in each group, and were challenged only by 10 mL·kg-1·d-1 lavage, and continuous dosing for 7 days. On the 8th day, rats were challenged at ambient temperature (41.0±0.5)?℃, relative humidity (10±1)% of the northwest in the special environment of artificial lab, placed in 0 (normal temperature), 50, 100 and 150 minutes respectively. The levels of CD19 and CD11b in peripheral blood of each rat were detected by flow cytometry instrument. Results With the extension of time in the simulated dry and heat environment, the level of CD11b in peripheral blood was gradually increased in each group, and the peak value was reached at 150 minutes, the NS control group, solvent control group and curcumin low, medium and high dose pretreatment groups were 0.346±0.013, 0.342±0.013, 0.342±0.012, 0.325±0.012, and 0.281±0.012, respectively. In each group, the level of CD19 was first increased and then decreased, reaching its peak value at 100 minutes, and the level of the NS control group, solvent control group and curcumin low, medium and high dose pretreatment groups were 0.586±0.010, 0.601±0.014, 0.684±0.009, 0.613±0.012 and 0.604±0.006, respectively. The level of CD11b in the curcumin medium and high dose pretreatment groups were significantly lower than those in the NS control group and solvent control group (50 minutes: 0.237±0.011, 0.188±0.006 vs. 0.283±0.009, 0.289±0.012;100 minutes: 0.260±0.010, 0.248±0.008 vs. 0.293±0.00

关 键 词：沙漠 干热环境 中暑 姜黄素 免疫 流式细胞术 CD11B CD19 

分 类 号：R594.12[医药卫生—内科学]