连翘药材、标准汤剂、中间体和配方颗粒的HPLC指纹图谱相关性  

Correlation on HPLC Fingerprint Spectra of Raw Herbs, Standard Decoction, Intermediates and Dispensing Granules of Forsythia Suspense

作　　者：曹静亚 李智宁[1,2] 李飞飞 陈玲[2,3] 魏悦 CAO Jingya;LI Zhining;LI Fei;CHEN Ling;WEI Yue(Henan Cogo Testing and International Standardizing Technology Co., Ltd., Zhengzhou 450002;Biological Developing Center of Henan, Zhengzhou 450002;Henan Plant Natural Products Development Engineering Research Center, Zhengzhou 450002)

机构地区：[1]河南科高中标检测技术有限公司,河南郑州450002 [2]河南省生物技术开发中心,河南郑州450002 [3]河南省植物天然产物开发工程技术研究中心,河南郑州450002

出　　处：《中国医药工业杂志》2019年第3期332-337,共6页Chinese Journal of Pharmaceuticals

基　　金：河南省科学院科研开发项目(18KF13003;18JK16014;190613035)

摘　　要：建立了连翘药材、标准汤剂、中间体和配方颗粒的HPLC指纹图谱,并对其相关性进行研究。采用Agilent Eclipse XDB-C_(18)反相色谱柱,以甲醇和水为流动相进行梯度洗脱,流速1.0 ml/min,柱温25℃,检测波长235 nm。连翘10批药材、标准汤剂、中间体及配方颗粒指纹图谱均呈现10个共有特征峰,呈良好的相关性。在10个共有峰中分析确认了6个化学成分,分别为forsythenside F(3号峰)、连翘酯苷Ⅰ(4号峰)、(+)-松脂醇-4-O-β-D-吡喃葡萄糖苷(5号峰)、连翘酯苷A(7号峰)、连翘苷(8号峰)及连翘脂素(10号峰)。连翘药材、标准汤剂、中间体和配方颗粒的主要化学成分组成基本相同,建立的HPLC指纹图谱方法可用于连翘配方颗粒生产全过程的质量控制。In this study, the HPLC fingerprint spectra of the raw herbs, standard decoction, intermediates and dispensing granules of Forsythia suspense were established. The correlations among them were analyzed. An Agilent Eclipse XDB-C18 RP column was used with methanol and water as the mobile phase with a linear gradient elution. The flow rate was 1.0 ml/min, the column temperature was 25 ℃ with the detection wavelength of 235 nm. There were ten common characteristic peaks in the fingerprint spectra of the ten batches of raw herbs, standard decoction, intermediates and dispensing granules of Forsythia suspense. The results presented a good correlation. Six peaks were identified from the ten common peaks, including forsythenside F(peak 3), forsythoside Ⅰ(peak 4),(+)-pinoresinol-4-O-β-Dglucopyranoside (peak 5), forsythoside A (peak 7), forsythin (peak 8) and phillygenin (peak 10). The main chemical compositions existing in the raw herbs, standard decoction, intermediates and dispensing granules of Forsythia suspense are basically the same. The established HPLC fingerprints can be used to control the quality in the whole production process of Forsythia suspense dispensing granules.

关 键 词：指纹图谱 连翘 药材 标准汤剂 中间体 配方颗粒 相关性 

分 类 号：R286.0[医药卫生—中药学]