长链非编码RNA IFNG-AS1 SNP与桥本甲状腺炎的易感性关联分析  

Association between lncRNA IFNG-AS1 SNP and Hashimoto′s thyroiditis

作　　者：易如海[1] 苏跃青[2] 曾赛凡[3] 赵淑好[1] 杨立勇[1] Yi Ruhai;Su Yueqing;Zeng Saifan;Zhao Shuhao;Yang Liyong(Endocrinology Department, The First Affiliated Hospital of Fujian Medical University, Diabetes Research Insititute of Fujian Province, Fuzhou 350005, China;Center of Neonatal Screening, Fujian Provincal Maternity and Children′s Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou 350000, China;Pathology Department, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China)

机构地区：[1]福建医科大学附属第一医院内分泌科,福建省糖尿病防治研究院,福州350005 [2]福建省妇幼保健院,福建医科大学附属医院,新生儿筛查中心,福州350000 [3]福建医科大学附属第一医院病理,福州350005

出　　处：《中华内分泌代谢杂志》2019年第2期105-110,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：福建省自然科学基金(2016J01542).

摘　　要：目的探讨IFNG基因相关的长链非编码RNA IFNG-AS1单核苷酸多态性(lncRNA IFNG-AS1 SNP)与桥本甲状腺炎(HT)易感性之间的关系。方法选取福建地区汉族HT患者70例和相应健康体检者109例为研究对象,应用TaqMan探针技术进行单核苷酸多态性位点(rs10878724、rs7980829和rs11177020)分型检测,采用实时荧光PCR法进行外周血IFNG-AS1和IFNG基因mRNA定量分析。结果与正常对照组比较,病例组除rs10878724的A等位基因频率和AA基因型显著性增高(P=0.01、P=0.003)外,rs7980829的T等位基因频率和TT基因型也显著性增高(P=0.026、P=0.011)。单体型分析显示:G-G-A降低HT发病风险(P=0.04),A-T-T增加HT的发病风险(P=0.01)。病例组的IFNG-AS1和基因mRNA表达量增加均具有统计学意义(P=0.001、P=0.013)。在病例组中,rs7980829的TT基因型和rs10878724的AA基因型的IFNG基因mRNA与其他基因型比较均有显著性增加(P=0.017,P=0.009)。结论lncRNA IFNG-AS1的SNP位点与IFNG基因mRNA和lncIFNG-AS1表达量有关联,可能是HT的易感基因。Objective To assess the association between three single nucleotide polymorphisms(SNPs)(rs10878724、rs7980829 and rs11177020) of lnc IFNG-AS1 and Hashimoto′s thyroiditis(HT) susceptibility. Methods TaqMan probe technology was used to genotype the selected SNPs in a total of 179 subjects, including 70 HT cases, and 109 controls. The expression levels of lnc IFNG-AS1 and IFNG were detected by SYBR-Green qRT-PCR. Results Compared with control, not only the A allele and AA genotype frequencies of rs10878724 were significantly different in group HT(P=0.01, P=0.003), but also the T allele and TT genotype frequencies of rs7980829 were significantly high in group HT. Haplotype analysis showed that the G-G-A decreased the risk of HT(P=0.04), while the haplotype of A-T-T incresed the risk of HT(P=0.01). The relative expression of both IFNG mRNA and lnc IFNG-AS1 were higher in group HT than in control(P=0.001, P=0.013). In HT patients, IFNG mRNA relative expression in both rs7980829-TT and rs1087872-TT were significantly higher than those of other genotypes(P=0.017, P=0.009). Conclusion The SNPs of Inc IFNG-AS1 were correlated with the expression levels of IFNG and lncRNA IFNG-AS1. Noncoding genes should be further screened as potential biomarkers in prediction of HT susceptibility.

关 键 词：IFNG基因 IFNG-AS1 长链非编码RNA 单核苷酸多态性 桥本甲状腺炎 

分 类 号：R581.4[医药卫生—内分泌]