哺乳动物雷帕霉素靶蛋白、核转录因子-κB、固醇调节元件结合蛋白2在子痫前期患者中的表达及意义  

Expression of mammalian target of rapamycin(mTOR),nuclear factor-κ B(NF-κB) and sterol regulatory element binding protein 2 (SREBP2) in serum and placentas among gravidas with preeclampsia

作　　者：杨天丽[1] 喻玲[1] 胡芸[1] 丁依玲[1] Yang Tianli;Yu Ling;Hu Yun;Ding Yiling(Department of Obstetrics and Gynecology,the Second Xiangya Hospital of Central South University,Changsha 410011,China)

机构地区：[1]中南大学湘雅二医院妇产科,长沙410011

出　　处：《中华围产医学杂志》2019年第3期186-193,共8页Chinese Journal of Perinatal Medicine

基　　金：湖南省自然科学基金青年基金项目(2018JJ3770).

摘　　要：目的探讨子痫前期患者外周血及胎盘组织中哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin, mTOR)、核转录因子-κB(nuclear factor-κ B, NF-κB)、固醇调节元件结合蛋白2(sterol regulatory element binding protein 2, SREBP2)的表达意义。方法选择2015年8月至2017年8月在中南大学湘雅二医院进行规律产前检查并剖宫产分娩的60例子痫前期患者,根据子痫前期分度标准分为重度子痫前期(severe preeclampsia, SPE)组(40例)和轻度子痫前期(mild preeclampsia, MPE)组(20例),并按照2∶1比例选择同期因头盆不称、胎位异常及社会因素行剖宫产终止妊娠的30例孕妇作为对照组。所有孕妇均采用酶联免疫吸附试验(enzyme linked immunosorbent assay, ELISA)法检测外周血mTOR、高密度脂蛋白-胆固醇(high density lipoprotein-cholesterol, HDL-C)、低密度脂蛋白-胆固醇(low density lipoprotein-cholesterol, LDL-C)、甘油三酯、总胆固醇浓度;采用蛋白质印迹法检测胎盘mTOR、磷酸化mTOR (phospho- mTOR, p-mTOR)、NF-κB、SREBP2蛋白表达。采用单因素方差分析、Bonferroni法或Dunnett's T3检验,以及Pearson相关分析进行统计分析。结果(1)SPE组和MPE组孕妇外周血mTOR[分别为(11 765.56±1 698.95)、(8 278.56±1 106.59)与(4 366.19±716.43) pg/ml]、LDL-C[分别为(7.81±1.90)、(4.11±0.75)与(2.42±0.45) mmol/L]水平均高于对照组(P值均<0.05),且SPE组均高于MPE组(P值均<0.05);SPE组和MPE组孕妇外周血HDL-C水平低于对照组[分别为(0.36±0.12)、(0.85±0.11)与(1.33±0.16) mmol/L],且SPE组低于MPE组(P值均<0.05)。SPE组孕妇外周血甘油三酯水平均高于MPE组及对照组[分别为(46.19±18.92)、(35.55±6.54)与(33.24±9.78) nmol/L,P值均<0.05],SPE组和MPE组孕妇外周血胆固醇水平均高于对照组[分别为(24.72±7.17)、(21.83±4.19)与(16.32±3.88)nmol/L,P值均<0.05 ]。(2)SPE组和MPE组孕妇胎盘mTOR(分别为0.52±0.09、0.38±0.08与0.24±0.05)、p-mTOR(分别为0.42±0.08、0.26Objective To investigate the level of mammalian target of rapamycin (mTOR) in serum and the expression of mTOR, nuclear factor-κ B (NF-κB) and sterol regulatory element binding protein 2 (SREBP2) in placenta among gravidas with preeclampsia. Methods From August 2015 to August 2017, 60 gravidas including 40 with severe preeclampsia (SPE) and 20 with mild preeclampsia (MPE) who underwent regular prenatal care and delivered by caesarean section were selected from the Second Xiangya Hospital of Central South University. According to the ratio of 2∶1, 30 gravidas who delivered through caesarean section due to cephalopelvic disproportion, abnormal fetal position or social factors during the same period were enrolled as the control group. Peripheral blood samples were obtained to determine the concentrations of serum mTOR, high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), triglyceride (TG) and total cholesterol (TC) by enzyme linked immunosorbent assay (ELISA). The expression of mTOR, phospho-mTOR (p-mTOR), NF-κB and SREBP2 in placenta were measured by Western blot. Clinical datas were statistically analyzed using one-way ANOVA, Bonferroni or Dunnett's T3 test, and Pearson's correlation analysis. Results (1) The serum levels of mTOR and LDL-C in the SPE and MPE group were both higher than that in the control group [mTOR:(11 765.56±1 698.95) and (8 278.56±1 106.59) vs (4 366.19±716.43) pg/ml;LDL-C:(7.81±1.90) and (4.11±0.75) vs (2.42±0.45) mmol/L, all P<0.05].Furthermore the serum levels of mTOR and LDL-C in the SPE group were both higher than those in the MPE group (both P<0.05). The serum level of HDL-C in the SPE and MPE group were lower than that in the control group [(0.36±0.12) and (0.85±0.11) vs (1.33±0.16) mmol/L, both P<0.05], and that in the SPE group was lower than that in the MPE group (P<0.05). Women in the SPE group showed higher TG level when comparing with the MPE and control group [(46.19±18.92) vs (35.55±6.54) and (33.24±9.78) nmol/L, both P<0.05

关 键 词：子痫前期 哺乳动物雷帕霉素靶蛋白 核转录因子-ΚB 胆固醇调节元件结合蛋白2 

分 类 号：R714.244[医药卫生—妇产科学]