人表皮黑素PIG1细胞株前列腺素E_2受体和前列腺素F_2α受体表达  

Expressions of prostaglandin E_2 and F_2α receptors in human epidermal melanocyte cell line PIG1

作　　者：王思涵 张贝[1] 毛子慧 于建斌[1] 曹靖[2] WANG Sihan;ZHANG Bei;MAO Zihui;YU Jianbin;CAO Jing(Department of Dermatology t the First A.ffiliated Hospital of Zhengzhou University,Zhengzhou 450052, China;Department of Human Anatomy , College of Basic Medical Science of Zhengzhou University , Zhengzhou 450001, China)

机构地区：[1]郑州大学第一附属医院皮肤科,郑州450052 [2]郑州大学基础医学院人体解剖学教研室,郑州450001

出　　处：《中华实用诊断与治疗杂志》2019年第3期221-223,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：河南省科技厅项目(182300410305)

摘　　要：目的探讨前列腺素(prostaglandin, PG)E_2受体、PGF_2α受体FP在人表皮黑素PIG1细胞株表达变化。方法取对数生长期人表皮黑素PIG1细胞株,采用免疫荧光染色法检测PGE_2受体包括EP1、EP2、EP3、EP4以及PGF_2α受体FP表达。结果 PIG1细胞株细胞膜和细胞质中均有EP1、EP2、EP3、EP4、FP阳性荧光染色。结论 PG通过EP1、EP2、EP3、EP4及FP调控人表皮黑素PIG1细胞功能。Objective To explore the expressions of prostaglandin E2 (PGE2) receptors and PGF2 a receptor FP in human epidermal melanocyte cell line PIG1. Methods The expressions of PGE2 receptors (EP1, EP2, EP3, EP4) and FP of PIG1 melanocytes in logar让hmic growth phase were detected by direct immunofluorescence staining. Results The positive fluorescent stainings of EP1, EP2, EP3, EP4 and FP were seen in the cytomembrane and cytoplasma of PIG1 melanocytes. Conclusion Prostaglandin modulates the function of PIG1 melanocytes by EP, EP2, EP3, EP4 and FP.

关 键 词：人表皮黑素PIG1细胞 前列腺素E2受体 前列腺素F2α受体 

分 类 号：R751[医药卫生—皮肤病学与性病学]