血清miR-181b及miR-210在妊娠期高血压疾病中表达及与炎性细胞因子的相关性  

Expressions of serum miR-181b and miR-210 in hypertensive disorders in pregnancy and its correlation with inflammatory cytokines

作　　者：胡际东[1] 温丽[1] 郭珍[1] 王丽丽[1] 周欢欢[1] HU Jidong;WEN Li;GUO Zhen;WANG Lili;ZHOU Huanhuan(Department of Obstetrics and Gynecology,Zhuozhou Hospital,Zhuozhou072750,China)

机构地区：[1]涿州市医院妇产科,河北涿州072750

出　　处：《中华实用诊断与治疗杂志》2019年第3期241-244,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：保定市科学计划与发展指导计划项目(16ZF049)

摘　　要：目的探讨妊娠期高血压疾病(hypertensive disorders in pregnancy, HDP)患者血清miR-181b、miR-210表达变化,分析其与白细胞介素(interleukin, IL)-1β、IL-6及肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)的关系。方法 HDP患者226例,其中妊娠期高血压(gestational hypertension, GH)60例(GH组)、轻度子痫前期(mild preeclampsia, MP)90例(MP组)、重度子痫前期(servere preeclampsia, SP)76例(SP组),同期健康孕妇60例为对照组。检测4组血清miR-181b mRNA、miR-210 mRNA相对表达量及IL-1β、IL-6及TNF-α水平;Pearson相关分析HDP患者血清miR-181b mRNA、miR-210 mRNA相对表达量与IL-1β、IL-6及TNF-α的相关性。结果对照组、GH、MP、SP组血清miR-181b mRNA相对表达量(1.42±0.65、5.73±1.60、10.94±4.28、17.13±8.16)、miR-210 mRNA相对表达量(0.84±0.13、3.62±1.05、4.74±1.36、7.92±2.17),IL-1β[(2.60±0.84)、(11.26±2.53)、(17.65±6.24)、(23.92±9.16)ng/L]、IL-6[(90.26±27.45)、(106.35±33.74)、(128.42±36.18)、(151.46±40.27)ng/L]及TNF-α[(12.40±2.28)、(19.25±4.42)、(35.84±6.53)、(64.73±9.50)ng/L]水平依次增高(P<0.05);HDP患者血清miR-181b mRNA相对表达量与IL-1β、IL-6、TNF-α呈正相关(r=0.694,P<0.001;r=0.735,P<0.001;r=0.846,P<0.001)、miR-210 mRNA相对表达量与IL-1β、IL-6、TNF-α呈正相关(r=0.548,P=0.003;r=0.718,P<0.001;r=0.815,P<0.001)。结论 HDP患者miR-181b及miR-210表达上调,且上调程度与HDP病情严重程度及IL-1β、IL-6、TNF-α水平变化相关,可能参与HDP的发生、发展。Objective To investigate the expressions of serum miR- 181b and miR-210 in patienis with hypertensive disorders in pregnancy (HDP )? and their correlations with interleukin ( IL )-ip, IL-6 and tumor n ecrosis factor-a (TNF a). Methods Totally 226 patients with HDP were divided into 60 patients with gestational hypertension (GH group), 90 patients with mild preeclampsia ( MP group) and 76 patients with severe preeclampsia ( SP group )? and another 60 healthy pregnant women in the same period were selected as control group. The relative expressions of serum miR 181b mRNA and miR-210 mRNA as well as the levels of 11" 10, IL-6 and TNF-a were detected in four groups. Pearson correlation method was used to analyze the correlation of serum miR-181b mRNA and miR-210 mRNA levels with IL-lp, IL-6 and TNF-a in HDP patients. Results The relative expressions of serum miR-181 b mRNA ( 1. 42 ± 0. 65. 5. 73± 1. 60, 10. 94 土 4.28, 17. 13土& 16), miR-210 mRNA (0.84 士 0. 13, 3.62±l.O5, 4. 74± 1. 36. 7.92 土 2. 17), IL-ip ((2. 60 士 0.84),(11. 26±2. 53),( 17. 65 ±6. 24),(23. 92 ±9. 16) ng L), IL-6 ((90. 26± 27. 45).( 106. 35 ± 33.74),(128. 42±36. 18),( 151. 46±40. 27) ng/L) and TNF-a ((12. 40 土 2.28),(19. 25±4. 42).(35. 84±6. 53).(64. 73±9. 50) ng/L) increased gradually in control group.(;H group. MP group and SP group in turns (PV0. 05). The relative expression of serum miR 181b mRNA was positively correlated with 1L-10. IL-6 and TNF-a in HDP patients (厂=0.694, PV0. 001;厂= 0.735, PV0. 001;厂= 0.846. PV0.()()1). and the relative expression of miR-210 mRNA waspositively correlated with IL-1β,IL-6and TNF-α(r=0.548,P=0.003;r=0.718,P<0.001;r=0.815,P<0.001). Conclusion The expressions of serum miR-181band miR-210are up-regulated in HDP patients along with the severity of HDP as well as the changes of IL-1β,IL-6and TNF-αlevels,and might be involved in the occurrence and development of HDP.

关 键 词：妊娠期高血压疾病 miR-181b MIR-210 白细胞介素-1Β 白细胞介素-6 肿瘤坏死因子-Α 

分 类 号：R714.246[医药卫生—妇产科学]