高效液相色谱法同时测定葛根散中葛根素、大豆苷的含量  

Simultaneous Determination of Puerarin and Daidzin in Gegen Powder by HPLC

作　　者：石金敏 张彩光 周灿 王佳 余文锋 余绪明 SHI Jin-min;ZHANG Cai-guang;ZHOU Can;WANG Jia;YU Wen-feng;YU Xu-ming(Department of Pharmacy,Shiyan People’s Hospital (The Affiliated People’s Hospital of Hubei University of Medicine),Shiyan 442000,China;School of Pharmacy,Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]十堰市人民医院(湖北医药学院附属人民医院)药学部,湖北十堰442000 [2]湖北医药学院药学院,湖北十堰442000

出　　处：《食品与药品》2019年第2期124-126,共3页Food and Drug

基　　金：十堰市科学技术研究与开发项目(编号:16Y36);十堰市科学技术研究与开发项目(编号:16Y40);湖北医药学院学生科研基金资助(编号:201810929064)

摘　　要：目的建立同时测定葛根散中葛根素和大豆苷含量的方法。方法色谱柱为Inertsil C_(18)柱(250 mm×4.6mm,5μm),乙腈-0.1%磷酸水溶液(10:90)为流动相,葛根素、大豆苷检测波长为250 nm,流速1.00 ml/min。结果葛根素、大豆苷质量浓度与峰面积呈良好线性关系,葛根素和大豆苷的线性范围分别是1.4～90μg/ml(r=1.0000),2.5～160μg/ml(r=1.0000),加样回收率分别为97.57%(RSD=1.75%)和99.64%(RSD=1.96%)。结论该方法快速、准确,适用于葛根散中葛根素和大豆苷的含量测定。Objective To develop an HPLC method for determination of puerarin and daidzin in Gegen Powder.Methods The Inertsil C18 column(250 mm×4.6 mm,5μm)was used.Acetonitrile-0.1%phosphonic acid(10:90)was used as the mobile phase.The flow rate was 1.00 ml/min.The detection wavelength was 250 nm.Results The linear range of puerarin and daidzin was 1.4-90μg/ml(r=1.0000),2.5-160μg/ml(r=1.0000),respectively.The average recoveries were 97.57%and 99.64%,respectively,with RSDs of 1.75%and 1.96%,respectively.Conclusion The established method is fast and accurate,which is suitable for the determination of puerarin and daidzin in Gegen Powder.

关 键 词：葛根散 葛根素 大豆苷 HPLC 

分 类 号：R927[医药卫生—药学]