Fabrication of high-capacity cation-exchangers for protein adsorption: Comparison of grafting-from and grafting-to approaches  

作　　者：Ming Zhao Run Liu Jian Luo Yan Sun Qinghong Shi 

机构地区：[1]Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China [2]Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China [3]State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

出　　处：《Frontiers of Chemical Science and Engineering》2019年第1期120-132,共13页化学科学与工程前沿（英文版）

基　　金：the National Natural Science Foundation of China (Grant Nos. 21476166 and 21236005);the Open Funding Project of the State Key Laboratory of Biochemical Engineering (No. 2014KF-03);the Tianjin Natural Science Foundation (15JCYBJC48500).

摘　　要：In this work, we have synthesized two polymer-grafted cation exchangers: one via the grafting-from approach, in which sulfopropyl methacrylate (SPM) is grafted through atom transfer radical polymerization onto Sepharose FF (the thus resulting exchanger is referred as Sep-g-SPM), and another via the grafting-to approach, in which the polymer of SPM is directly coupled onto Sepharose FF (the thus resulting exchanger is called as Sep-pSPM). Protein adsorption on these two cation exchangers have been also investigated. At the same ligand density, Sep-g-SPM has a larger accessible pore radius and a smaller depth of polymer layer than Sep-pSPM, due to the controllable introduction of polymer chains with the regular distribution of the ligand. Therefore, high-capacity adsorption of lysozyme and γ-globulin could be achieved simultaneously in Sep-g-SPM with an ionic capacity (IC) of 308 mmol·L^-1. However, Sep-pSPM has an irregular chain distribution and different architecture of polymer layer, which lead to more serious repulsive interaction to proteins, and thus Sep-pSPM has a lower adsorption capacity for γ-globulin than Sep-g-SPM with the similar IC. Moreover, the results from protein uptake experiments indicate that the facilitated transport of adsorbed γ-globulin occurs only in Sep-pSPM and depends on the architecture of polymer layers. Our research provides a clear clue for the development of high-performance protein chromatography.

关 键 词：polymer-grafted ionic EXCHANGER GRAFTING technique protein adsorption atom transfer radical polymerization γ-globulin 

分 类 号：TQ[化学工程]