慢性间歇性缺氧引起大鼠舌下神经核超微结构损伤的初步研究  

Preliminary study on ultrastructural damage of hypoglossal nucleus in rats caused by chronic intermittent hypoxia

作　　者：朱华 曹芮 李佳宸[1] 史雅文[1] 殷敏[1] Zhu Hua;Cao Rui;Li Jiachen;Shi Yawen;Yin Min(Department of Otolaryngology, the First Affiliated Hospital of NMU,Nanjing 210029;Nanjing Agricultural University Hospital, Nanjing 210095, China)

机构地区：[1]南京医科大学第一附属医院耳鼻咽喉科,江苏南京210029 [2]南京农业大学医院,江苏南京210095

出　　处：《南京医科大学学报（自然科学版）》2019年第2期187-190,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：中央高校基本科研业务费专项资金(KYYL201703);江苏省高层次卫生人才"六个一工程"(LGY2017 070)

摘　　要：目的:通过大鼠造模,探讨慢性间歇性缺氧(chronic intermittent hypoxia,CIH)状态下,大鼠舌下神经元超微结构损伤的情况。方法:将12只雄性SD大鼠(8周龄,体重180～200 g)随机均分为CIH组和常氧对照组,每组各6只。CIH组6只大鼠饲养于氧浓度5%～21%之间循环的低氧舱中,每天8 h,持续35 d;对照组大鼠饲养箱的氧浓度保持在21%,持续35 d。5周后在电镜下观察两组大鼠舌下神经元超微结构损伤的情况。结果:电镜结果显示常氧组大鼠舌下神经元细胞膜完整,核膜平滑,染色质均匀,细胞器丰富。而CIH组细胞膜呈不连续性;细胞核膜内陷,胞核呈不规则形;神经元内出现水肿空泡化;线粒体肿胀,外膜模糊不清,有破损情况;高尔基体和滑面内质网出现肿胀扩张现象;还可见脂褐素。结论:慢性间歇性缺氧可诱导大鼠舌下神经核损伤,可在电镜下观察到舌下神经核一系列超微结构变化。Objective:To study chronic intermittent hypoxia(CIH)-induced ultrastructural damage of hypoglossal motor nucleus in modeling rat. Methods:Twelve adult male Sprague-Dawley(SD)rats(8 weeks old,weighing 180-200 g)were randomly and meanly devided into two groups(the CIH group and the control group),with 6 rats in each group. The control group was fed in atmosphere environment cabins with 21% oxygen. And the rats of CIH group were fed in low oxygen cabins for 8 hours a day for 35 days with 5% to21% oxygen. We study the ultrastructural damage of hypoglossal motor nucleus in rat under electron microscope. Results:Electron microscopy showed that the cell membrane of the hypoglossal neurons in the normoxic group was continuous,the nuclear membrane was smooth,the chromatin was homogeneous,and the organelles were abundant. In the CIH group,the cell membrane was discontinuous,the shape of nucleus was irregular,and its membrane was invaginated. There were some areas of edema vacuolization in the neurons. The mitochondria are swollen,and its outer membrane is ambiguous;the Golgi body and the smooth endoplasmic reticulum appear to be swollen and dilated;and lipofuscin is also observed. Conclusion:CIH can induce sublingual nucleus damage in rats,so that a series of ultrastructural changes in the hypoglossal nucleus can be observed under electron microscope.

关 键 词：慢性间歇性缺氧 舌下神经核 超微结构 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]