电针“神门”“三阴交”对失眠大鼠下丘脑室旁核能量代谢的影响  

Effect of electroacupuncture at “Shenmen”(HT7) and “Sanyinjiao”(SP6) on energy metabolism in paraventricular nucleus of hypothalamus of insomnia rats

作　　者：诸毅晖[1] 杨翠花 何林熹 乔玲 成词松[2] ZHU Yi-hui;YANG Cui-hua;HE Lin-xi;QIAO Ling;CHENG Ci-song(College of Acupuncture-moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;College of Basic Medicine,Chengdu Univcrsity of Traditional Chinese Medicine,Chengdu 611137)

机构地区：[1]成都中医药大学针灸推拿学院,成都610075 [2]成都中医药大学基础医学院,成都611137

出　　处：《针刺研究》2019年第3期170-175,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81273851)

摘　　要：目的:观察电针"神门""三阴交"对失眠大鼠下丘脑室旁核(PVN)能量代谢的影响,探讨电针治疗失眠症的中枢调节机制。方法:SD大鼠随机分为正常组、模型组和电针组,每组22只。模型组和电针组采用慢性束缚法束缚15d复制失眠模型。电针组电针"神门""三阴交",每日1次,每次15min,治疗5d。用24h小动物自发活动数据采集系统检测大鼠24h自发活动量,以负重力竭游泳实验检测大鼠负重力竭游泳时间,采用蛋白免疫印迹法检测PVN内的腺苷酸活化蛋白激酶(AMPK)表达,酶联免疫法检测PVN内的乙酰辅酶A(Ac-CoA)、钠-钾三磷酸腺苷酶(Na+-K+-ATPase)含量以及血浆皮质酮(CORT)含量,透射电镜观察PVN的细胞结构。结果:与正常组比较,模型组大鼠白天活动量增多、夜晚活动量减少(P<0.05),负重力竭游泳时间缩短(P<0.05);PVN内AMPK表达增强(P<0.05)、Ac-CoA和Na^+-K^+-ATPase含量降低(P<0.05),血浆CORT含量降低(P<0.05);电镜下可见模型大鼠PVN内线粒体肿胀,核糖体部分消失。电针后,与模型组比较,电针组大鼠白天活动量减少、夜晚活动量增多(P<0.05),负重力竭游泳时间延长(P<0.05);PVN内AMPK表达减弱(P<0.05)、Ac-CoA和NA^+-K^+-ATPase含量升高(P<0.05),血浆CORT含量升高(P<0.05);PVN内线粒体仅轻度肿胀。结论:电针"神门""三阴交"对失眠大鼠下丘脑室旁核能量代谢有良好调整作用,通过调节下丘脑室旁核AMPK表达、Ac-CoA和Na^+-K^+-ATPase含量,电针可以有效改善失眠及失眠后疲劳症状。Objective To observe the effect of electroacupuncture(EA)at"Shenmen"(HT7)and"Sanyinjiao"(SP6)on energy metabolism in paraventricular nucleus(PVN)of hypothalamus in insomnia rats,so as to explore its mechanism underlying improvement of insomnia.Methods A total of 66 SD rats(half male and half female)were randomized into 3 groups:normal control,model and EA groups(n=22 per group).The insomnia model was established by binding the rat for at least 4 h(step increase of 30 min per day),once daily for 15 days.EA(5 Hz/25 Hz,0.5-1.0 mA)was applied to unilateral HT7 and SP6 for 15 min,once daily for 5 days.The rats’ spontaneous activities during day and night were recorded by using the ClockLab Data Collection and Analysis System,and the duration of exhausted swimming was detected by using load-bearing endurance swimming test.The expression of adenosine 5′-monophosphate(AMP)-activated protein kinase(AMPK)of PVN tissue was assayed by Western blot,and the contents of acetyl coenzyme A(Ac-CoA)and Na^+-K^+adenosine triphosphatase(Na^+-K^+-ATPase)in the PVN tissue,and corticosterone(CORT)in plasma were assayed by ELISA.Changes of the ultrastructure of PVN cells were observed by transmission electron microscope.Results After modeling,the rats’ daytime and nocturnal locomotor activities were significantly increased and decreased,respectively(P<0.05),and the duration of exhausted swimming was considerably shortened in the model group compared with that of the normal control group(P<0.05).The expression level of AMPK protein in the PVN was obviously up-regulated(P<0.05),and the contents of Ac-CoA and Na^+-K^+-ATPase in PVN and CORT in plasma were markedly decreased in the model control group relevant to the normal group(P<0.05).After EA intervention,the increased daytime locomotion and the decreased nocturnal activities,the shortened duration of exhausted swimming,the up-regulated expression of AMPK,and the decreased Ac-CoA,Na^+-K^+-ATPase and CORT contents were all reversed in the EA treated rats relevant to those of the ins

关 键 词：失眠 电针 下丘脑室旁核 腺苷酸活化蛋白激酶 乙酰辅酶A 钠-钾三磷酸腺苷酶 

分 类 号：R245.97[医药卫生—针灸推拿学]