电针“内关”预处理对心肌缺血再灌注损伤大鼠血清代谢物的影响  

Serum metabolic profile involving protective effect of “Neiguan”(PC6)-electroacupuncture preconditioning in rats with myocardial ischemia reperfusion injury

作　　者：陈美琳[1] 王超[1] 谭成富 刘薇薇[1] 郭礼娜 杜琳[1] 唐雅妮 李姣兰 朱鼎铭 章薇[2] CHEN Mei-lin;WANG Chao;TAN Cheng-fu;LIU Wei-wei;GUO Li-na;DU Lin;TANG Ya-ni;LI Jiao-lan;ZHU Ding-ming;ZHANG Wei(College of Acupunclure-moxibustion and Massage, Hunan University of Chinese Medicine,Changsha 410007 , China;Department of Acupuncture and Moxibustion of the First Affiliated Hospital,Hunan University of Chinese Medicine,Changsha 410007)

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410007 [2]湖南中医药大学第一附属医院针灸科,长沙410007

出　　处：《针刺研究》2019年第3期176-182,共7页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81072868);湖南省中医药管理局课题(No.201521);湖南省高校创新平台开放基金项目(No.15k093)

摘　　要：目的:基于核磁共振氢谱技术(1 H NMR)观察电针"内关"穴预处理对心肌缺血再灌注损伤(MIRI)大鼠血清代谢物及其代谢通路的影响,从代谢组学角度探讨电针预处理对MIRI大鼠产生保护作用的机制。方法:雄性SD大鼠48只随机分为正常组、模型组、电针内关组(内关组)、电针合谷组(合谷组),每组12只。采用结扎冠状动脉左前降支40min后恢复血流1h制备MIRI大鼠模型。两电针组造模前每天予电针相应穴位30min,连续7d。模型制备成功后,收集各组大鼠血清,利用~1 H NMR进行代谢物检测,利用最小二乘分析、正交偏最小二乘分析进行模式识别。结果:在造模后,模型组、电针组大鼠心电图均表现出T波高耸,R波与T波间无明显ST段。再灌注后T波下降超过0.2mV,无明显ST段。与正常组比较,模型组大鼠血清代谢模式发生明显改变,乙酰乙酸、乳酸、肌酸、丙三醇、葡萄糖升高,丙氨酸、谷氨酰胺、甘油磷酸胆碱、磷酸胆碱降低。与模型组比较,内关组大鼠血清代谢模式发生明显改变,表现为葡萄糖上升,亮氨酸、异亮氨酸、缬氨酸、三羟基丁酸、乳酸、醋酸盐、丙酮、乙酰乙酸、丙酮酸、谷氨酰胺、肌酸、丙三醇下降;合谷组与模型组代谢模式无显著差异。结论:电针"内关"预处理明显改变了MIRI大鼠糖代谢、丙酮酸代谢、氨基酸代谢、酮体代谢、能量代谢的模式,电针"内关"可能通过调节丙酮酸、氨基酸、酮体、能量等对MIRI大鼠起到一定的预保护作用。Objective To observe the effect of"Neiguan"(PC6)-electroacupuncture(EA)preconditioning on serum metabolites in myocardial ischemia-reperfusion injury(MIRI)rats,so as to reveal its mechanism underlying improvement of ischemic myocardium from metabonomics.Methods A total of 48 male SD rats were randomly divided into control,model,EA"Neiguan"(PC6)and EA"Hegu"(LI4)groups(n=12 rats/group).Rats of the control group were just banded on animal boards for 30 min,once daily for 7 days.The MIRI model was established by occlusion of the left anterior descending branch of the left coronary artery for 40 min,followed by reperfusion for 1 h,and rats of the model group were also banded as those in the control group.Before modeling,EA(10 Hz/50 Hz,1 mA)was applied to bilateral"Neiguan"(PC6)and"Hegu"(LI4)for 30 min,once daily for 7 successive days.After the treatment,serum samples were collected to be analyzed by proton nuclear magnetic resonance(1 H NMR)spectroscopy.The orthogonal partial least squares discriminate analysis(PLS-DA)was employed to distinguish the serum differential metabolic profile of rats in different groups and identify potential biomarkers.Results After modeling,the ECG of model group and electroacupuncture groups showed T wave towering,and there was no obvious ST segment between R wave and T wave.The T wave decreased more than 0.2 mV after reperfusion,and there was no obvious ST segment.Compared with the control group,MIRI induced significant changes of metabolites in the serum including increase of acetoacetate acid,lectic acid,creatine,glycerol and glucose,and decrease of alanine,glutamine,glycerophosphoryl choline and phosphorylcholine.In comparison with the model group,PC6-EA preconditioning induced significant changes,including an increase of glucose,and a decrease of leucine,isoleucine,valine,3-hydroxybutyric acid,lactate,acetate,acetone,acetoacetate acid,pyruvic acid,glutamine,creatine and glycerol.There is no significant difference in metabolic patterns between"Hegu"group and model group.Metabolic path

关 键 词：电针预处理 心肌缺血再灌注损伤 代谢组学 核磁共振氢谱技术 

分 类 号：R245.97[医药卫生—针灸推拿学]