针刺通过抑制肝脏氧化应激改善腹型肥胖型非酒精性脂肪肝病大鼠的肝脏脂质代谢  

Acupuncture improves hepatic lipid metabolism by suppressing oxidative stress in obese nonalcoholic fatty liver disease rats

作　　者：王海英 梁翠梅[1] 崔静文 潘良[1] 胡慧[1] 方红娟 WANG Hai-ying;LIANG Cui-mei;CUI Jing-wen;PAN Liang;HU Hui;FANG Hong-juan(Department of Acupunc ture-moxibustion, Dongfang Hospital, Beijing University of Chinese Medicine,Beijing 100078, China;Department of Endocrinology, Beijing Tian-tan Hospital,Capital Medical University,Beijing 100070)

机构地区：[1]北京中医药大学东方医院针灸科,北京100078 [2]首都医科大学附属天坛医院内分泌科,北京100070

出　　处：《针刺研究》2019年第3期189-194,215,共7页Acupuncture Research

基　　金：北市市科学技术委员会"首都临床特色应用研究"专项课题(No.Z141107002514079);北京市中医管理局中医药"3+3"薪火传承工程<杨甲三名家研究室>;首都医科大学基础-临床科研合作课题(No.17JL34);北京市医院管理局青苗人才计划资助(No.QML20160501)

摘　　要：目的:探讨针刺对腹型肥胖型非酒精性脂肪肝病(NAFLD)大鼠脂质代谢的影响及其机制。方法:雄性SD大鼠随机分为空白组11只和造模组30只,高脂饮食喂养12周,建立腹型肥胖合并NAFLD模型。空白组和造模组分别随机选取3只验证模型。最终造模成功的16只大鼠随机分为模型组8只和针刺组8只。针刺组予自制鼠袋内固定,针刺双侧"带脉"穴,深度约为4～5mm,接电针,疏密波,2Hz/15Hz,1.5mA,每次20min,隔日1次,干预8周。测量各组大鼠体质量、腹围、内脏脂肪及肝脏湿重,全自动生化仪检测大鼠血清总胆固醇(TC)、血清甘油三酯(TG),谷丙转氨酶(ALT)、谷草转氨酶(AST),CT扫描检测腹部内脏脂肪分布,肝组织匀浆测定丙二醇(MDA)、总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-PX),苏木精-伊红(HE)染色观察肝脏病理形态学改变。结果:针刺前及针刺4、8周时,模型组大鼠体质量、腹围、内脏脂肪质量、肝脏湿重较空白组明显升高(P<0.001,P<0.05)。针刺4、8周末,与模型组比较,针刺组大鼠的体质量、腹围明显下降(P<0.05),针刺组内脏脂肪、肝脏湿重较模型组明显降低(P<0.05)。针刺8周末,与空白组比较,模型组大鼠血清ALT、AST、TC、TG明显升高(P<0.001,P<0.01),与模型组比较,针刺组血清ALT、AST、TC、TG水平均显著下降(P<0.05),CT结果可见腹部内脏脂肪分布减少,肝脏病理形态学可见肝脏脂肪变性程度改善。与空白组比较,模型组大鼠肝组织MDA含量明显升高(P<0.001),T-SOD、GSH-PX活性降低(P<0.001);与模型组比较,针刺组MDA含量下降、T-SOD及GSH-PX活性升高(P<0.01)。结论:针刺可以减轻腹型肥胖型NAFLD大鼠体质量、腹围,减少腹部内脏脂肪堆积,改善肝脏脂质代谢,其机制可能与抑制氧化应激有关。Objective To investigate the effect of acupuncture of"Daimai"(GB26)on abdominal fat accumulation,lipid metabolism and hepatic oxidative stress in abdominal obese non-alcoholic fatty liver disease(NAFLD)rats.Methods male SD rats were divided into 3 groups:normal diet(normal,n=8),high fat diet control(model)and acupuncture(n=8/group in the latter2 groups).The abdominal obese NAFLD model was established by feeding the rats with high fat diet for 12 weeks.EA(2 Hz/15 Hz,1.5 mA)was applied to bilateral GB26 for 20 min,once every other day for 8 weeks.Rats of the model group were also restrained for 20 min as those in the EA group.The body mass and abdominal circumference were measured weekly,the isolated adipose tissues around the bilateral kidney and epididymis and the fresh liver were weighed.The contents of serum cholesterol(TC),triglyceride(TG),alanine transaminase(ALT),glutamic oxaloacetic aminotransferase(AST)were detected by using an automatic biochemical analyzer.The abdominal visceral fat distribution was acquired by CT scanning.The oxidative stress indexes of the homogenated liver tissues,such as malondialdehyde(MDA)was assayed using sodium thiobarbital(TBA)method,and the activity of total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-PX)were assayed by using hydroxylamine method and colorimetric method respectively.The histopathological changes of the liver were observed after staining with hematoxylin-eosin(HE).Results Following modeling,the body mass and waist circumference,visceral fat weight of bilateral kidneys and testis(visceral fat weight),liver weight,serum ALT,AST,TG and TC and liver MDA contents,were significantly higher in the model group(P<0.001,P<0.05),while hepatic T-SOD and GSH-PX activity was considerably lower in the model group than those in the normal group(P<0.001).After acupuncture intervention,the levels of all the above-mentioned indexes(modeling induced both increase and decrease)were reversed relevant to the model group(P<0.05,P<0.01).The results of CT scanning showed th

关 键 词：针刺 腹型肥胖 非酒精性脂肪肝病 氧化应激 脂质代谢 

分 类 号：R245.97[医药卫生—针灸推拿学]