不同针灸疗法对老年雄性大鼠下丘脑-睾丸中生殖激素调控系统相关基因表达的影响  

Warm needle moxibustion,mild moxibustion and electroacupuncture interventions have an antiaging effect possibly by regulating hypothalamus-pituitary-testis axis in aged male rats

作　　者：马琳[1] 李学智[1,2] 姚太万 吴佳鹏 席小芳 傅燕 刘嘉 MA Lin;L1 Xue-zhi;YAO Tai-wan;WU Jia-peng;XI Xiao-fang;FU Yan;LIU Jia(College of Traditional Chinese Medicine,Chongqing Medical University,Chongqing 400016,China;Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Treat me nt of Metabolic Diseases, Chongqing 400016;Chongqing City Vocational College of Management ,Chongqing 40001;Experimental Teaching Management Center,Chongqing Medical University, Chongqing 400016;Chongqing Ji-ai Reproductive Health Guidance and Consultation Center,Chongqing 400016)

机构地区：[1]重庆医科大学中医药学院,重庆400016 [2]重庆市中医药防治代谢性疾病重点实验室,重庆400016 [3]重庆城市管理职业学院,重庆400016 [4]重庆医科大学实验教学管理中心,重庆400016 [5]重庆集爱生殖保健指导咨询中心,重庆400016

出　　处：《针刺研究》2019年第3期200-204,219,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81303036);重庆市基础科学与前沿技术研究项目(No.cstc2017jcyjAX0213);重庆市渝中区科技计划项目(No.20160108)

摘　　要：目的:探讨不同针灸疗法对老年大鼠血清性激素水平的影响及延缓衰老作用的基因调控途径。方法:SD老年大鼠随机分为老年组、温针灸组、温和灸组、电针组、药物组,每组8只,另取SD青年大鼠8只作为青年组。温针灸组、温和灸组及电针组取"肾俞""关元"穴治疗,每日1次,每次15min;药物组给予腹部皮下注射丙酸睾酮注射液(7mg·kg^(-1)·3d^(-1))。各组均治疗8周。ELISA法检测大鼠血清睾酮(T)、促性腺激素释放激素(GnRH)含量,实时荧光定量PCR法检测大鼠下丘脑中KISS-1、G蛋白耦联受体(GPR)54、RF酰胺相关肽-3(RFRP-3)、GPR147和睾丸中GnRH-R、GPR54、GPR147mRNA的表达。结果:老年组大鼠血清T、GnRH含量,下丘脑中KISS-1、GPR54、RFRP-3、GPR147mRNA表达及睾丸组织中GnRH-R、GPR54mRNA表达低于青年组(P<0.05),睾丸组织GPR147mRNA表达量高于青年组(P<0.05)。与老年组比较,温针灸组、温和灸组、电针组、药物组血清T含量升高(P<0.05),温针灸组、温和灸组、电针组血清GnRH含量,下丘脑KISS-1、GPR54、RFRP-3、GPR147 mRNA表达升高(P<0.05),睾丸组织GnRH-R、GPR54mRNA表达量升高,GPR147mRNA表达量降低(P<0.05)。温针灸组、温和灸组、电针组各指标改善均优于药物组(除血清T含量外,P<0.05)。结论:温针灸、温和灸和电针均能显著提高老年大鼠血清T水平,温针灸、温和灸、电针与丙酸睾酮补充疗法对于血清T水平的提高程度接近,但药物并不能明显激活GnRH合成分泌通路,故温针灸、温和灸和电针更适合在临床中作为长期治疗的方法。Objective To observe the effect of warm needle moxibustion(WNM),mild moxibustion and electroacupuncture(EA)of"Shenshu"(BL23)and"Guanyuan"(CV4)on serum gonadal hormone levels and related gene expression in the hypothalamus and testis in aged rats,so as to explore their underlying mechanisms in anti-aging.Methods Forty male SD male rats were randomly divided into aged control,WNM,mild moxibustion,EA and medication groups(n = 8 in each group),and other 8 male young adult SD rats were used as the youth control group.WNM(acupuncture plus moxibustion),mild moxibustion or EA(2 Hz/100 Hz,1 mA)was separately applied to CV4 and bilateral BL23 for 15 min,once daily,5 days a week for 8 weeks.Rats of the medication group received subcutaneous injection of testosterone propionate injection(7 mg/kg),once every 3 days,and those of the aged and youth groups received the same dose of 0.9% NaCl solution for 8 weeks.The levels of serum testosterone(T)and gonadotropin-releasing hormone(GnRH)were measured by ELISA.The expression of Kisspeptin(KISS-1),G protein-coupled receptors 54(GPR54),RFamide-related peptide-3(RFRP-3)and GPR147 mRNAs in the hypothalamus and GnRH-R,GPR54 and GPR147 mRNAs in the testis tissues were detected by quantitative real-time PCR.Results The levels of serum T and GnRH,and the expressions of KISS-1,GPR54,RFRP-3 and GPR147 mRNAs in the hypothalamus and GnRH-R and GPR54 mRNAs in the testis in the aged rats were significantly lower than those in the youth rats(P<0.05),while the expression level of GPR147 mRNA of the testis tissue was obviously higher in the aged group than that in the youth group(P<0.05).Following the treatment,the lowered serum T and GnRH contents,the down-regulated expression levels of hypothalamic KISS-1,GPR54,RFRP-3 and GPR147 mRNAs and testicle GnRH-R and GPR54 mRNAs,as well as the up-regulated testicle GPR147 mRNA expression in the aged rats were reversed in the WNM,mild moxibustion and EA groups(not the medication group except serum T)in comparison with the aged control group(P<0.05).The th

关 键 词：温针灸 电针 温和灸 抗衰老 性腺激素 下丘脑-垂体-性腺轴 

分 类 号：R245[医药卫生—针灸推拿学]