黄芩苷对过敏性鼻炎大鼠免疫因子及IL-33/ST2信号通路的影响  

Effect of Baicalin on immune factor and IL-33/ST2 signaling pathway in rats with allergic rhinitis

作　　者：马志跃[1] 李彬[1] 冯勇[1] 余晓旭[1] 樊建刚[1] 何刚[1] Ma Zhiyue;Li Bin;Feng Yong;Yu Xiaoxu;Fan Jiangang;He Gang(Sichuan Academy of Medical Sciences Sichuan People's Hospital,Chengdu,Sichuan 610072,China)

机构地区：[1]四川省人民医学科学院·四川省人民医院耳鼻咽喉头颈外科,四川成都610072

出　　处：《医药前沿》2019年第5期37-39,共3页Journal of Frontiers of Medicine

基　　金：四川省成都市科技局课题(2015-HM01-00172-SF).

摘　　要：目的:观察黄芩苷对过敏性鼻炎(A R)大鼠免疫因子及I L-33/S T2信号通路的影响.方法:24只健康S D大鼠随机分为空白组(A)、模型组(B)、黄芩苷低剂量组(C)、黄芩苷高剂量组(D),除A组外,其余3组建立大鼠A R模型.每组于致敏第15d后开始给药,C、D组分别以50、100m g/k g黄芩苷尾静脉注射,A、B组灌胃等量0.9%生理盐水,连续14d,处死,采样待检.HE染色观察鼻黏膜组织病理学变化;ELISA检测血清IgE、IL-4、INF-γ含量;RT-PCR检测鼻黏膜IL-33、ST2、NF-kB mRNA表达.结果:D组较B组IgE、IL-4、IL-33、ST2、NF-kB表达均明显降低,IFN-γ含量明显升高(P<0.01).结论:黄芩苷可调节AR免疫因子及IL-33/ST2信号通路,对AR有治疗作用.Objective To observe the effect of baicalin on immune factors and IL-33/ST2 signaling pathway in allergic rhinitis (AR) rats. Methods Twenty-four healthy SD rats were randomly divided into blank group (A), model group (B), baicalin low-dose group (C), and baicalin high-dose group (D). Rat AR models were established in the remaining 3 groups except group A. Each group was started after 15 days of sensitization. Groups C and D were injected intravenously with 50 and 100 mg/kg baicalin. Groups A and B were intragastrically administered with 0.9% normal saline for 14 days and the rats were sacrificed and samples were taken for examination. The histopathological changes of nasal mucosa were observed by HE staining. Serum IgE, IL-4 and INF-γ levels were detected by ELISA. The expressions of IL-33, ST2 and NF-kB mRNA in nasal mucosa were detected by RT-PCR. Results The expression of IgE, IL-4, IL-33, ST2 and NF-kB in group D was significantly lower than that in group B, and the content of IFN-γ was significantly increased (P<0.01). Conclusions Baicalin can regulate AR immune factor and IL-33/ST2 signaling pathway and has therapeutic effect on AR.

关 键 词：过敏性鼻炎 黄苓苷 免疫因子 大鼠 

分 类 号：R969.4[医药卫生—药理学]