太湖氨化功能菌群的分布及其有机氮降解条件  

Distribution of Ammonifying Bacteria in Taihu Lake and Conditions of Degrading Organic Nitrogen

作　　者：王红 阮爱东 徐洁[1,2] WANG Hong;RUAN Aidong;XU Jie(State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering,Hohai University,Nanjing 210098,China;College of Hydrology and Water Resources,Hohai University,Nanjing 210098,China)

机构地区：[1]河海大学水文水资源与水利工程科学国家重点实验室,南京210098 [2]河海大学水文水资源学院,南京210098

出　　处：《河南科学》2019年第3期439-446,共8页Henan Science

基　　金：国家自然科学基金项目(51378175)

摘　　要：为探究富营养化湖泊水体微生物对有机氮的去除,用最大可能数(MPN)法测定了太湖氨化菌的分布,并进行了氨化菌的分离纯化、筛选以及生理生化分析、有机氮降解条件及效果研究.结果表明,太湖水体中氨化菌数量普遍偏高,从东部沿岸区域到西部沿岸区氨化菌数量逐步增多.太湖4处采样点氨化菌数量随着底泥深度的增加呈下降-上升-下降的规律.氨化菌的培养最佳条件为28℃、pH 7.2、盐度0.004 mol/L.筛选出的6株氨化菌AB-1～AB-6,它们对有机氮的降解率分别为36%、69%、67%、71%、83%、56%,AB-5菌株对有机氮的降解能力最大.本研究丰富了降解有机氮菌种资源,可为该菌在湖泊微生物驱动氮元素转化提供参考,为解决太湖水体中氮素污染问题,改善太湖水质环境提供科学依据.In order to explore the removal of organic nitrogen by microorganisms in eutrophic lake,the distribution of ammonifying bacteria in Taihu Lake was determined by the Most Probable Number(MPN)method,and the isolation,purification,screening and physiological and biochemical analysis of ammonifying bacteria,degradation conditions and effects of organic nitrogen were carried out.It showed that the number of ammonifying bacteria was generally high in Taihu Lake,and it gradually increased from the eastern coastal area to the western coastal area.With the increase of sediment depth,the number of ammonifying bacteria at the four sampling sites of Taihu Lake with decreases,increases and decreases.The optimum conditions for cultivation of ammoniating bacteria were 28 ℃,pH 7.2 and salinity 0.004 mol/L.Ammonifying bacteria AB-1 to AB-6 were screened to degrade organic nitrogen,which were 36%,69%,67%,71%,83% and 56%,respectively.AB-5 strain had the greatest ability to degrade organic nitrogen.This study enriches the resources of organic nitrogen degrading bacteria,provides a reference for the transformation of nitrogen elements driven by microorganisms in lakes,and provides a scientific basis for solving the problem of nitrogen pollution in Taihu Lake and improving the water quality and environment of Taihu Lake.

关 键 词：氨化菌 太湖 MPN法 降解效果 

分 类 号：X524[环境科学与工程—环境工程]