不同药物雾化吸入对SD大鼠肺组织的影响  

Effect of different drugs inhalation on SD rats lung tissue

作　　者：赫倩 唐荣国 李凡彩[2] 王秀娟[3] 李斌[1] 宋晓丹[1] 欧维琳[1] He Qian;Tang Rongguo;Li Fancai;Wang Xiujuan;Li Bin;Song Xiaodan;Ou Weilin(Department of Pediatrics,the Affiliated Hospital of Guilin Medical College,Guilin 541001,Guangxi Zhuang Autonomous Region,China;Department of Pathology,the Affiliated Hospital of Guilin Medical College,Guilin 541001,Guangxi Zhuang Autonomous Region,China;Department of Clinical Laboratory,the Affiliated Hospital of Guilin Medical College,Guilin 541001,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]桂林医学院附属医院儿科,广西桂林541001 [2]桂林医学院附属医院病理科,广西桂林541001 [3]桂林医学院附属医院检验科,广西桂林541001

出　　处：《中华实用儿科临床杂志》2019年第4期295-299,共5页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(81360248);广西自然科学基金(2014GXNSFAA118171).

摘　　要：目的通过研究不同药物雾化吸入对SD大鼠肺组织的影响,探索非雾化剂型药物用于雾化的安全性。方法无特定病原体(SPF)级健康雄性SD大鼠40只随机分为空白对照组、9g/L盐水组、沙丁胺醇组、定喘汤组、双黄连组、庆大霉素组、丹参组、二氧化硅组共8组(5只/组)。每天雾化2次,持续56d。取外周血、支气管肺泡灌洗液(BALF)进行细胞计数与分类。左肺中叶切片HE染色观察肺组织病理变化并计数尘细胞数目,免疫组织化学法检测CD163表达水平。结果1.外周血白细胞计数:空白对照组[(3.96±0.36)×10^9/L]、9g/L盐水组[(4.66±0.58)×10^9/L]、沙丁胺醇组[(4.06±0.86)×10^9/L]、定喘汤组[(8.98±1.08)×10^9/L]、双黄连组[(7.10±0.88)×10^9/L]、庆大霉素组[(6.14±0.89)×10^9/L]、丹参组[(9.84±2.33)×10^9/L]、二氧化硅组[(8.99±2.48)×10^9/L],8组间比较差异有统计学意义(F=14.530,P<0.05);空白对照组、9g/L盐水组、沙丁胺醇组间比较差异均无统计学意义(均P>0.05)。BALF白细胞计数:空白对照组[(2.16±1.04)×10^9/L]、9g/L盐水组[(3.94±0.67)×10^9/L]、沙丁胺醇组[(4.36±1.15)×10^9/L]、定喘汤组[(14.58±2.93)×10^9/L]、双黄连组[(19.68±6.29)×10^9/L]、庆大霉素组[(11.74±1.03)×10^9/L]、丹参组[(44.75±10.8)×10^9/L]、二氧化硅组[(53.54±14.25)×10^9/L],8组间比较差异有统计学意义(F=40.616,P<0.05);空白对照组、9g/L盐水组、沙丁胺醇组间比较差异均无统计学意义(均P>0.05)。BALF淋巴细胞计数:空白对照组[(18.70±9.00)×10^8/L]、9g/L盐水组[(36.01±5.99)×10^8/L]、沙丁胺醇组[(38.95±11.69)×10^8/L]、定喘汤组[(132.70±26.94)×10^8/L]、双黄连组[(173.56±57.6)×10^8/L]、庆大霉素组[(106.60±16.76)×10^8/L]、丹参组[(340.63±70.97)×10^8/L]、二氧化硅组[(495.63±131.95)×10^8/L],8组间比较差异有统计学意义(F=41.980,P<0.05);空白对照组、9g/L盐水组、沙丁胺醇组间比较差异均无统计学意义(均P>0.05)。2.Objective To have SD rats inhaled with different drugs,and observe their lung pathological change of lungs through light microscopy,in order to evaluate the safety of different drugs inhaled by natural rats.Methods A total of 40 rats were randomly divided into 8 groups,and every group had 5 rats,including blank control groups,9 g/L saline group,Salbutamol group,Dingchuantang group,Shuanghuanglian group,Centamicin group,Danshen group,Silicon dioxide group,twice a day,last 56 days totally.Then,blood and bronchoalveolar lavage fluid were collected and analyzed for cell count,percent of each type of cell,to measure the severity of the inflammation.Additionally,histopathology revealed the lung′s pathological change and the number of dust cell;while immunohistochemistry revealed CD163 responding.Results(1)White blood cell count:blank control group(3.96±0.36)×10^9/L,9 g/L saline group(4.66±0.58)×10^9/L,Salbutamol group(4.06±0.86)×10^9/L,Dingchuantang group(8.98±1.08)×10^9/L,Shuanghuang-lian group(7.10±0.88)×10^9/L,Centamicin group(6.14±0.89)×10^9/L,Danshen group(9.84±2.33)×10^9/L,Silicon dioxide group(8.99±2.48)×10^9/L,and comparative analysis of the 8 groups had significant difference(F=14.530,P<0.05);the differences among blank control group,9 g/L saline group and Salbutamol group were not significant(all P>0.05).White cell count in BALF:blank control group(2.16±1.04)×10^9/L,9 g/L saline group(3.94±0.67)×10^9/L,Salbutamol group(4.36±1.15)×10^9/L,Dingchuantang group(14.58±2.93)×10^9/L,Shuanghuanglian group(19.68±6.29)×10^9/L,Gentamicin group(11.74±1.03)×10^9/L,Danshen group(44.75±10.8)×10^9/L,Silicon dioxide group(53.54±14.25)×10^9/L,and comparative analysis of the 8 groups had significant difference(F=40.616,P<0.05);the differences among blank control group,9 g/L saline group and Salbutamol group were not significant(all P>0.05).Lymphocyte count in BALF:blank control group(18.70±9.00)×10^8/L,9 g/L saline group(36.01±5.99)×10^8/L,Salbutamol group(38.95±11.69)×10^8/L,Dingchuantang

关 键 词：雾化吸入 肺损伤 安全性 

分 类 号：R725.6[医药卫生—儿科]