促胰岛β细胞增生的黄酮类化合物的活体筛选  

In vivo screening stimulators of pancreatic β-cell hyperplasia from flavonoids

作　　者：王冲 林春雨 吴志强[1] 李明玉 WANG Chong;LIN Chunyu;WU Zhiqiang;LI Mingyu(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China;School of Pharmaceutical Sciences,Xiamen University,Xiamen 361102,China)

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]厦门大学药学院,福建厦门361102

出　　处：《药学研究》2019年第3期135-139,共5页Journal of Pharmaceutical Research

基　　金：国家自然科学基金(No.81670709);福建省自然科学基金(No.2017J01145);中央高校基本科研业务费(No.20720170104;20720180044)

摘　　要：目的利用斑马鱼为模型进行黄酮类药物筛选,以期发现促进胰岛β细胞增生的药物。方法对生长5 d的斑马鱼胚胎进行黄酮类药物处理,并进行β细胞计数,筛选出能够促进β细胞数量增加的药物,并进一步分析其促进β细胞增加是源自于已有β细胞的复制或前体细胞的分化。结果淫羊藿素(Icaritin)能够增加胰岛β细胞的数量,并明显促进β细胞的复制。同时,我们发现有几个黄酮类化合物具有降血糖的作用。结论淫羊藿素能够明显促进β细胞的自我复制从而促进β细胞的增生。Objective To find compounds that stimulate pancreatic β-cell hyperplasia by using zebrafish model for drug screening of flavonoids.Methods 5 d (days post fertilization) zebrafish larvae were treated with flavonoids for 24 hours and the number of β-cell was counted to select the compounds that could increase its number.Further analysis of its promotion of β-cell growth was derived from the replication of existing β-cell or the differentiation of precursor cells.Results:Icaritin can increase zebrafish β-cell number and the increase was contributed by β-cell self-proliferation.Moreover,we found that several flavonoids can decrease the glucose level of zebrafish larvae.Conclusion Icaritin can promote the β-cell hyperplasia by stimulating its proliferation.

关 键 词：斑马鱼 糖尿病 药物筛选 黄酮类药物 胰岛Β细胞 

分 类 号：R285.5[医药卫生—中药学]