羟考酮依赖或戒断反应时大鼠脊髓c-fos蛋白表达的变化  

Changes in expression of c-fos protein in spinal cord in a rat model of oxycodone dependence or withdrawal response

作　　者：颜杏 罗科 邵佳丽 张小艳 夏月峰 邬娇 孙辉平 刘景诗[2] 杨金凤 Yan Xing;Luo Ke;Shao Jiali;Zhang Xiaoyan;Xia Yuefeng;Wu Jiao;Sun Huiping;Liu Jingshi;Yang Jinfeng(Department of Anesthesiology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University,Changsha 410078,China;Department of Anesthesiology,Hunan Provincial People's Hospital,Changsha 410005,China)

机构地区：[1]中南大学湘雅医学院附属肿瘤医院麻醉科,长沙市410078 [2]湖南省人民医院麻醉科,长沙市410005

出　　处：《中华麻醉学杂志》2018年第12期1456-1459,共4页Chinese Journal of Anesthesiology

基　　金：湖南省卫生健康委员会科研计划项目(B2014-106).

摘　　要：目的评价羟考酮依赖或戒断反应时大鼠脊髓c-fos蛋白表达的变化。方法SPF级成年雄性SD大鼠30只,6~8周龄,体重180~220g,采用随机数字表法分为3组(n=10):生理盐水组(NS组)、羟考酮依赖组(OD组)和羟考酮戒断组(OW组)。OD组和OW组背部皮下注射羟考酮,共5d,剂量依次为2、3、4、5、6mg/kg,每日3次(8:00/15:00/22:00),建立羟考酮依赖模型。NS组背部皮下注射等容量生理盐水。于给药前3d及每天末次给药后30min时测定机械缩足反应阈(MWT)。OW组在第5天末次注射羟考酮8h后腹腔注射纳洛酮4mg/kg,诱发羟考酮戒断反应。观察给予纳洛酮或生理盐水后15min内NS组与OW组戒断反应评分及体重变化幅度。OD组于第5天末次给药1h时、NS组和OW组于第5天给予生理盐水或纳洛酮1h时,取脊髓组织,采用Westernblot法测定脊髓c-fos蛋白的表达。结果与NS组比较,OD组和OW组给药后1、2d时MWT升高,脊髓c-fos蛋白表达上调,OW组戒断症状评分增高,体重变化幅度增大(P<0.05);与OD组比较,OW组c-fos蛋白表达下调(P<0.05)。结论大鼠羟考酮依赖及戒断反应可能与脊髓c-fos蛋白有关,依赖时表达上调,戒断反应时表达下调。Objective To evaluate the changes in the expression of c-fos protein in the spinal cord in a rat model of oxycodone dependence or withdrawal response. Methods Thirty SPF adult male Sprague-Dawley rats, aged 6-8 weeks, weighing 180-220 g, were divided into 3 groups (n=10 each) using a random number table method: normal saline group (group NS), oxycodone dependence group (group OD), and oxycodone withdrawal group (group OW). In OD and OW groups, oxycodone was injected subcutaneously in back, 5 days in total, with the dose of 2, 3, 4, 5 and 6 mg/kg in turn, 3 times a day (8: 00/15: 00/22: 00). The equal volume of normal saline was given instead in group NS.The mechanical paw withdrawal threshold was measured at 3 days before administration and 30 min after the last administration every day.The oxycodone withdrawal was induced by intraperitoneal injection of naloxone 4 mg/kg at 8 h after the last administration of oxycodone on 5th day in group OW.The withdrawal response scores and range of weight changes were recorded within 15 min after giving naloxone or normal saline in NS and OW groups.Spinal cord tissues were collected at 1 h after the last administration on 5th day in group OD and at 1 h after giving normal saline or naloxone on 5th day in NS and OW groups for determination of the expression of c-fos protein by Western blot. Results Compared with group NS, the mechanical paw withdrawal threshold was significantly increased on 1 and 2 days after administration, and the expression of c-fos protein in the spinal cord was up-regulated in OD and OW groups, and withdrawal response scores were significantly increased, and the range of weight change was increased in group OW (P<0.05). The expression of c-fos protein was significantly down-regulated in group OW as compared with group OD (P<0.05). Conclusion Oxycodone dependence or withdrawal response may be related to the expression of c-fos protein in the spinal cord of rats, and the expression is up-regulated during oxycodone dependence, while down-regulated during ox

关 键 词：径可酮 阿片相关性障碍 原癌基因蛋白质C-FOS 脊髓 

分 类 号：R614[医药卫生—麻醉学]