蓝莓采后病害病原菌分离鉴定及其拮抗菌筛选  

Pathogen identification of rotten blueberry fruit in post-harvest and screening of antagonistic bacteria

作　　者：杨蕾[1,2] 伍建榕 邓佳[3] 陆燕元 王芳[1] YANG Lei;WU Jianrong;DENG Jia;LU Yanyuan;WANG Fang(Key Laboratory of State Forestry Administration for Biodiversity Conservation in Southwest China, Southwest Forestry University;Key Laboratory of Forest Disaster Warning and Control in Yunnan Higher Education Institutions;Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Kunming, Yunnan 650224, China)

机构地区：[1]西南林业大学国家林业局西南地区生物多样性保育重点实验室 [2]云南省高校森林灾害预警控制重点实验室 [3]西南山地森林资源保育与利用省部共建教育部重点实验室,云南昆明650224

出　　处：《福建农林大学学报（自然科学版）》2019年第2期143-147,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：西南林业大学科技创新基金(C16029);西南林业大学木棉纤维人工林产业化培育省级创新团队项目;国家自然科学基金项目(31360198;31860208);云南省一流学科建设项目(51600625);云南省高校基础研究产业化项目(2016CYH14)

摘　　要：从西南林业大学大棚蓝莓种植基地内采集样本,并从采后腐烂果实中分离出8株真菌,成功鉴定出2株为青霉菌(Penicillium digitatum)和枝孢菌(Cladosporium tenuiussimum).从健康蓝莓果实、叶片和根际土壤中共分离到17株细菌,根据16S rRNA序列同源性,分别归入9个细菌属.通过检测其对蓝莓果腐病病原真菌的抑制活性,筛选出达到显著差异的拮抗细菌5株,其中3株为芽孢杆菌,2株为短芽孢杆菌;5株拮抗细菌对P.digitatum的抑菌率为67.6%～90.6%,其中3株拮抗细菌对C.tenuiussimum的抑菌率达90.7%～92.6%.菌株BS-3对P.digitatum和C.tenuiussimum的抑菌效果均在90%以上,有潜在生防利用价值.Eight fungal strains isolated from rotten blueberry harvested in the greenhouse in Southwest Forestry University were characterized and identified as Penicillium digitatum and Cladosporium tenuiussimum. Meanwhile, another 17 bacterial strains were isolated from healthy fruit, leaf and rhizosphere soil of blueberry and they were identified belonging to 8 genera based on 16S rRNA sequence homology analysis. Among them, 5 strains were found to have antagonistic effect on P.digitatum, with an efficacy of 67.6%-90.6%, and 3 strains had significantly higher antagonistic effect on C.tenuiussimum, with an efficacy of 90.7%-92.6%. These strains were identified as genus Bacillus. Among them, strain BS-3 showed high antagonism against both P.digitatum and C.tenuiussimum, with control efficacy being above 90%, which was very likely to be used as a biocontrol agent against post-harvest disease of blueberry.

关 键 词：蓝莓 果腐 病原菌鉴定 拮抗细菌 

分 类 号：S663.9[农业科学—果树学]