APOA4/C3基因簇及APOB基因多态性与新疆哈萨克族血脂异常的关系  

Relationship of the APOA4/C3 gene cluster and APOB gene polymorphisms with dyslipidemia in Kazakhs of Xinjiang

作　　者：张伟[1] 马晓磊 张凌云 周婷[1] 蒋松柏 廖新慧 王岳 吴静 黄刚[1] Zhang Wei;Ma Xiaolei;Zhang Lingyun;Zhou Ting;Jiang Songhai;Liao Xinhui;Wang Yue;Wu Jing;Huang Gang(the First Affiliated Hospital School of Medicine,Shihezi University,Shihezi,Xinjiang 832002,China;the First Hospital of Shijiazhuang,Shijiazhuang,Hebei 050000,China;People's Hospital of Wenquan County,Bole,Xinjiang 833500,China;People's Hospital of Tacheng City,Tacheng,Xinjiang 834700,China;People's Hospital of Shawan-County,Shihezi,Xinjiang 832100,China)

机构地区：[1]石河子大学第一附属医院,新疆石河子832002 [2]石家庄市第一医院,河北石家庄050000 [3]博州温泉县人民医院,新疆博乐833500 [4]塔城市人民医院,额敏塔城834700 [5]沙湾县人民医院,新疆石河子832100

出　　处：《石河子大学学报（自然科学版）》2018年第5期587-594,共8页Journal of Shihezi University(Natural Science)

基　　金：新疆兵团卫生科技计划(WSJ201268);新疆兵团医药卫生专项(2012BA052)

摘　　要：目的研究新疆哈萨克族APOA4/C3(Apolipoprotein A4/C3,APOA4/C3)基因簇和APOB(Apolipoprotein B,APOB)基因与血脂异常的相关性。方法采用病例对照研究,按照纳入和排除标准随机纳入哈萨克族血脂异常组268例、对照组243例作为研究对象。运用连接酶检测反应(Ligase Detection Reaction,LDR)技术进行单核苷酸多态(Single Nucleotide Polymorphism,SNP)分型。采用SPSS22.0统计软件包和SHEsis软件对数据资料行统计学处理和分析。结果(1)APOA4 rs5104、APOC3 rs734104、rs5128、APOB rs693四个SNP位点符合Hardy-Weinbreg平衡(P均>0.05)。(2)两组对比,APOA4 rs5104、APOC3 rs734104、rs5128、APOB rs693基因型和等位基因频率明显不同(P均<0.05)。(3)APOA4 rs5104 AG、GG或AG+GG基因型携带者患血脂异常的风险分别是AA基因型携带者的1.727、2.220、1.808倍,G等位基因携带者的血脂异常的风险是A等位基因的1.594倍。APOC3 rs734104 CT或CT+CC基因型携带者患血脂异常的风险分别是TT基因型携带者的1.574、1.621倍,C等位基因携带者的血脂异常的风险是T等位基因的1.457倍。APOC3 rs5128 CG、CC或CG+CC基因型携带者患血脂异常的风险分别是GG基因型携带者的1.917、2.135、1.959倍,C等位基因携带者的血脂异常的风险是G等位基因的1.680倍。APOB rs693 CT或CT+TT基因型携带者患血脂异常的风险分别是CC基因型携带者的0.408、0.399倍,T等位基因携带者的血脂异常的风险是C等位基因的0.443倍。(4)单倍型为ATGT者,患血脂异常的风险明显较低,差异有统计学意义(P﹤0.05),而GCCC患血脂异常的风险明显升高,差异有统计学意义(P﹤0.05)。结论新疆哈萨克族APOA4/C3基因簇及APOB基因多态性与血脂异常有关,进一步的研究仍是必要的。Objective To explore the relationship between polymorphisms of the APOA4/C3(apolipoprotein A4/C3, APOA4/C3)gene cluster and APOB(apolipoprotein B, APOB) gene with dyslipidemia in Kazakhs of Xinjiang, China. M ethods By random sampling method, 268 dyslipidemia patients were recruited as dyslipidemia group and 243 non-dyslipidemia subjects were included as control group in Kazakhs based on the inclusion and exclusion criteria. Genotyping of single nucleotide polymorphism(SNP) was performed by using Ligase detection reaction(LDR). SPSS 22.0 statistical software package was used for statistical data processing and analysis, and SHEsis software was used for the analysis of haplotypes. R esults The four SNPs of rs5104 of APOA4, rs734104 and rs5128 of APOC3, rs693 of APOB in control group conformed to Hardy-Weinberg’s equilibrium.Frequencies of rs5104, rs734104, rs5128, rs693 genotypes and alleles were different between dyslipidemia and the control group(all P<0.05). For rs5104, risk of dyslipidemia of G allele was 1.594 times as much as A allele. AG, GG and AG+GG were 1.727,2.220 and 1.808 times as much as AA to get dyslipidemia, respectively. For rs734104, risk of dyslipidemia of C allele was 1.457 times as much as T allele. CT and CT+CC were 1.574 and 1.621 times as much as TT to get dyslipidemia. For rs5128, risk of dyslipidemia of C allele was 1.680 times as much as G allele. Compared with GG genotype, CG, CC and CG+CC were 1.917,2.135 and 1.959 times, more likely to get dyslipidemia. For rs693, risk of dyslipidemia of T allele was 0.443 times as much as C allele. CT, and CT+TT were 0.408, 0.399 times as much as CC to get dyslipidemia, respectively. The risk of dyslipidemia with haplotype ATGT was lower than control group(P<0.05). The risk of dyslipidemia with haplotype GCCC was higher than control group(P<0.05). Conclusion The polymorphisms of APOA4/C3 gene cluster and APOB gene is related to dyslipidemia in Kazakhs, Xinjiang. Further investigation is needed.

关 键 词：APOA4/C3基因簇 APOB基因 血脂异常 哈萨克族 

分 类 号：R589.2[医药卫生—内分泌]