光照周期对尼罗罗非鱼幼鱼昼夜节律调节的作用  

Effects of light cycle on the circadian rhythm of juvenile Nile tilapia Oreochromis niloticus

作　　者：刘曾宇 周志成 吕为群[1,2,3] LIU Zengyu;ZHOU Zhicheng;LU Weiqun(Nationial Demonstration Center for Experimental Fisheries Science Eduration,Shanghai Ocean University,Shanghai 201306,China;International Research Center for Marine Biosciences,Ministry of Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Researces,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [2]上海海洋大学科技部海洋生物科学国际联合研究中心,上海201306 [3]上海海洋大学水产种质资源发展与利用教学重点实验室,上海201306

出　　处：《上海海洋大学学报》2019年第2期180-189,共10页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31572599;31072228;41376134)

摘　　要：为了研究尼罗罗非鱼幼鱼游泳行为在不喂食时是否存在昼夜节律和光照周期的调节作用,设计了光周期为光照(L)∶黑暗(D)=12 h∶12 h,持续的黑暗(DD),持续的光照(LL),光周期为L∶D=6 h∶6 h和光周期为L∶D=2 h∶2 h。结果表明:(1)光周期为L∶D=12 h∶12 h时,尼罗罗非鱼具有明显的昼夜节律,昼夜节律周期为(24. 3±0. 2) h;(2)尼罗罗非鱼的昼夜节律在持续的黑暗和光照下仍然存在,分别为(25. 1±1. 1) h和(25. 6±1. 0) h;(3)光周期为L∶D=6 h∶6 h时,尼罗罗非鱼仍具有明显的昼夜节律(12. 6±0. 5) h;(4)在光周期为L∶D=2 h∶2 h时,尼罗罗非鱼的昼夜游泳行为仍具有明显的昼夜节律,节律周期为(4. 0±2. 0) h。这些结果表明,尼罗罗非鱼具有以24 h为周期的内源性生物钟,但相比与外源性光照调控,内源性的生物钟对罗非鱼的调控较弱,外源性的光照周期才是调节尼罗罗非鱼幼鱼昼夜行为节律的主要因素。In order to study the circadian rhythm of juvenile Nile tilapia(Oreochromis niloticus),constant photoperiods were designed with 12 h in the light following 12 h in the darkness(12L:12D),6 h in the light following 6 h in the darkness(6L:6D)and 2 h in the light following 2 h in the darkness(2L:2D),respectively.The results showed that:(1)when the photoperiod was 24 h,the Nile tilapia showed circadian rhythm,and its circadian rhythm was(24.3±0.2)h;(2)the circadian rhythms of the Nile tilapia still existed under sustained darkness and illumination,which were(25.1+1.1)h and(25.6+1)h respectively;(3)when the photoperiod was 12 h,the Nile tilapia still showed an obvious circadian rhythm that was(12.6±0.5)h;(4)when the photoperiod was 4 h,the swimming behavior of Nile tilapia still had an obvious circadian rhythm,the rhythm was(4.0±2.0)h.These results indicated that the Nile tilapia had endogenous circadian clock with 24 h cycle,however,compared with exogenous light regulation,endogenous circadian clock is relatively weak in tilapia.Exogenous light cycle is the main factor regulating the circadian rhythm of juvenile Nile tilapia.

关 键 词：尼罗罗非鱼 光照 行为 昼夜节律 

分 类 号：S917[农业科学—水产科学]