云南省艾滋病患者分离新生隐球菌的药物敏感性检测与分析  

Detection and analysis of antimicrobial susceptibility of Cryptococcus neoformans isolated from patients with acquired immunodeficiency syndrome in Yunnan Province

作　　者：樊红丽 高丽 毛小琴 张米[1] 李正伦 杨翠先 FAN Hong-li;GAO Li;MAO Xiao-qin;ZHANG Mi;LI Zheng-lun;YANG Cui-xian(Department of Clinical Laboratory,AIDS Care Center,Yunnan Provincial Infectious Disease Hospital,Kunming 650301,China;Medical School of Kunming University of Science and Technology,Kunming 650500,China;Department of Clinical Laboratory,First People’s Hospital of Yunnan Province,Kunming 650032,China)

机构地区：[1]云南省传染病专科医院/艾滋病关爱中心检验科,云南昆明650301 [2]昆明理工大学医学院,云南昆明650500 [3]云南省第一人民医院检验科,云南昆明650032

出　　处：《中国感染控制杂志》2019年第4期340-343,共4页Chinese Journal of Infection Control

基　　金：2017年度云南省医疗卫生单位内设研究机构科研项目(2017NS122)

摘　　要：目的了解云南省艾滋病患者分离新生隐球菌的药物敏感性情况。方法采用比色微量稀释法,对2007年8月—2018年7月云南省传染病医院住院艾滋病患者标本分离的304株新生隐球菌进行药敏试验,检测6种常用抗隐球菌药物的敏感性。结果药敏结果显示:两性霉素B、5-氟胞嘧啶、氟康唑、伏立康唑、伊曲康唑及泊沙康唑MIC_(50)分别为1、2、4、0.03、0.06、0.12μg/mL,MIC_(90)分别为1、4、8、0.06、0.12、0.25μg/mL,几何均数分别为0.75、2.32、3.28、0.039、0.053、0.09μg/mL。在6种抗真菌药物中,新生隐球菌对伏立康唑和两性霉素B的耐药率为1.32%,5-氟胞嘧啶的耐药率为0.33%,未发现对伊曲康唑和氟康唑产生耐药的菌株。结论云南省艾滋病患者分离的新生隐球菌对不同药物敏感性有差异,可为临床选药提供参考。Objective To investigate antimicrobial susceptibility of Cryptococcus neoformans(C.neoformans)isolated from patients with acquired immunodeficiency syndrome(AIDS)in Yunnan Province.Methods 304 strains of C.neoformans isolated from AIDS patients in Yunnan Infectious Diseases Hospital between August 2007 and July 2018 were performed antimicrobial susceptibility testing by colorimetric microdilution method,susceptibility to 6 commonly used anti-cryptococcal agents was detected.Results Antimicrobial susceptibility testing results showed that MIC 50 of amphotericin B,5-fluorocytosine,fluconazole,voriconazole,itraconazole,and posaconazole were 1,2,4,0.03,0.06,and 0.12μg/mL respectively,and the MIC 90 were 1,4,8,0.06,0.12,and 0.25μg/mL respectively,with geometric mean values of 0.75,2.32,3.28,0.039,0.053,and 0.09μg/mL respectively.Among 6 antifungal agents,resistance rate of C.neoformans to voriconazole and amphotericin B was 1.32%,and to 5-fluorocytosine was 0.33%,no strain was found to be resistant to itraconazole and fluconazole.Conclusion C.neoformans isolated from AIDS patients in Yunnan Province have different susceptibility to different antimicrobial agents,which can provide reference for clinical antimicrobial choice.

关 键 词：艾滋病 人类免疫缺陷病毒 新生隐球菌 药敏试验 

分 类 号：R512.91[医药卫生—内科学] R446.5[医药卫生—临床医学]