基于叶片显微及亚显微结构的新疆扁桃10个主栽品种抗旱性综合评价  

Comprehensive evaluation of drought resistance of 10 main cultivars of almond(Amygdalus communis L.)in Xinjiang by means of leaf microstructure and ultrastructure

作　　者：木巴热克.阿尤普 艾沙江.买买提 郭春苗[1] 徐叶挺[1] 买买提依明.阿有甫 龚鹏[1] 杨波[1] Mubareke·Ayoupu;Aishajiang·Maimaiti;GUO Chunmiao;XU Yeting;Maimaitiyiming·Ayoufu;GONG Peng;YANG Bo(Institute of Horticulture,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,Xinjiang,China;Xinjiang Uygur Autonomous Region forestry ecological monitoring station,Urumqi 830091,Xinjiang,China)

机构地区：[1]新疆农业科学院园艺作物研究所,乌鲁木齐830091 [2]新疆维吾尔自治区林业生态监测总站,乌鲁木齐830091

出　　处：《果树学报》2019年第3期347-358,共12页Journal of Fruit Science

基　　金：新疆农业科学院青年基金项目(xjnkq-2017002);上海合作组织科技伙伴计划项目(2017E01025);国家自然科学基金项目(31860538)

摘　　要：【目的】从叶片形态解剖角度综合评价新疆10个扁桃(Amygdalus communis L.)主栽品种的抗旱性,为扁桃抗旱性评价指标体系的建立提供理论依据。【方法】通过光学和扫描电子显微镜观察叶片显微及亚显微结构相关20项指标并进行生物统计学分析。【结果】所有品种叶片均形成不同程度的旱生结构特征,角质层、栅栏细胞和维管系统均较发达,有含晶细胞和粘液细胞分布、气孔只分布于下表皮等。通过主成分分析,选取主脉厚度、叶比面积、叶肉细胞紧实度和气孔密度4项代表性指标。隶属函数评价结果表明,其抗旱能力由大到小排序为S14> S3> M7> M8>M1> M2> S12> S1>S9> S11。【结论】初步确定S14和S3的抗旱能力较强,而S9和S11的抗旱能力较弱,美国品种均属于中度抗旱类型。【Objective】The almond(Amygdalus communis L.)is one of the four most famous nuts in the world.It has a long history of cultivation, and has important nutritional and ecological value. As one of the origin of Almond in the world, Southern Xinjiang is rich in almond resistant resources. In this study, the drought resistant capacity of 10 different almond cultivars including 6 main local cultivars and 4 main American introduced cultivars collected from the Southern Xinjiang were evaluated by means of leaf microstructure and ultrastructure characteristics in order to provide a theoretical basis for the establishment of evaluation index system for drought resistance of almond,and to help understanding the drought resistant mechanism of almond from the anatomy aspects.【Methods】Fully-expanded matured leaves on one–year old twigs were sampled from 10 different almond cultivars on local peach rootstock from the southern Xinjiang. Leaf samples were fixed in FAA and 2.5% glutaraldehyde separately. Paraffin sections were made. At least 4 different leaves were used for cultivar, and the mesophyll cells structure and main vein structure were observed under the light microscopy;the scanning electronic microscopy was used for observing the ultrastructure of epidermis(4 different leaves for each cultivar, 2 of them used for observing upper epidermis, other 2 for observing lower epidermis). Besides of the anatomical structures, the SLA(specific leaf area) value of each cultivar was also calculated at least from 20 representative leaves. Except for the regular leaf anatomical indicators, crystal cell density(number of crystal cells per um2 mesophyll tissue), mucous cell distributed thickness(measured at least from 8 different points) and cell porosity ratio(ratio of intercellular space at mesophyll cells of the paraxial surface to the total area of mesophyll cells of the paraxial surface) etc., xerophytic plant related several leaf structural features were quantified. Thickness of cuticle, epidermis, palisade cell and tota

关 键 词：扁桃主栽品种 叶片解剖结构 抗旱性 主成分 隶属函数 

分 类 号：S662.9[农业科学—果树学]