敲除ABRO1不影响小鼠肝巨噬细胞重建  

Knockout of ABRO1 does not affect the reconstruction of hepatic macrophages

作　　者：汪煜 张文 张洁 任广明 杨晓明[1,2,3] 尹荣华 WANG Yu;ZHANG Wen;ZHANG Jie;REN Guang-ming;YANG Xiao-ming;YIN Rong-hua(Anhui Medical University, Hefei 230032, China;Institute of Radiation Medicine, Academy of Military MedicalSciences, Academy of Military Sciences, Beijing 100850, China;Institute of Lifeomics, Academy of Military MedicalSciences,Academy of Military Sciences, Beijing 102206, China;Department of Pharmaceutical Engineering, School ofChemical Engineering and Technology,Tianjin University, Tianjin 300072 , China)

机构地区：[1]安徽医科大学,合肥230032 [2]军事科学院军事医学研究院辐射医学研究所,北京100850 [3]军事科学院军事医学研究院生命组学研究所,北京102206 [4]天津大学化工学院制药工程系,天津300072

出　　处：《军事医学》2018年第10期731-735,共5页Military Medical Sciences

基　　金：蛋白质组学国家重点实验室合作研究课题(SKLPK201404)

摘　　要：目的建立一种高效清除并重建肝巨噬细胞的方法,检测敲除ABRO1对肝巨噬细胞重建的影响。方法8周龄CD45. 1亚型C57BL/6J小鼠,尾静脉注射氯氟松(clophosome)清除肝巨噬细胞,钴源照射后,移植Abro1+/+或Abro1-/-小鼠(CD45. 2)骨髓,8周后分离小鼠肝非实质细胞,流式分析检测肝巨噬细胞的重建效率。结果常规骨髓移植后,肝脏中单核来源巨噬细胞(CD11bhiF4/80lo)重建率高达90%,但Kupffer细胞(CD11bintF4/80hi)的重建率只有约10%;提前注射氯氟松后再进行骨髓移植,肝脏中单核来源巨噬细胞和Kupffer细胞的重建率都>90%。利用氯氟松清除肝巨噬细胞后再进行骨髓移植的方法,检测发现敲除ABRO1并不影响肝巨噬细胞的重建。结论通过采用尾静脉注射氯氟松联合骨髓移植的方法,成功建立了一种安全、高效清除并重建肝内巨噬细胞的方法,敲除ABRO1并不影响肝巨噬细胞的重建。Objective To establish an efficient method for removing and reconstructing hepatic macrophages,and detect whether ABRO1 knockout affects the reconstruction of hepatic macrophages. Methods Eight-week-old CD45. 1+C57 BL/6 J mice were injected with clophosome to remove liver macrophages. After cobalt irradiation,bone marrow of Abro1+/+or Abro1-/-( CD45. 2) was transplanted into CD45. 1+mice. Subsequently,after 8 weeks of bone marrow transplantation,the clearance and reconstruction efficiency of hepatic macrophages were detected by flow cytometry. Results In the cobalt irradiation group,the removal and reconstruction efficiency of CD11 bhiF4/80 locells was up to 90%,but that of CD11 bintF4/80 hicells was only 10%. In clophosome scavenging and cobalt irradiation group, the removal and reconstruction efficiency of CD11 bhiF4/80 locells and CD11 bintF4/80 hicells was both up to 95%. Using clophosome scavenging plus cobalt irradiation methods,we found that ABRO1 knockout did not affect the reconstruction of hepatic macrophages. Conclusion We have established a safe and efficient method for reconstructing hepatic macrophages by using the combination of clophosome clearance and cobalt irradiation. ABRO1 knockout does not affect the reconstruction of hepatic macrophages.

关 键 词：ABRO1 肝细胞 巨噬细胞 KUPFFER细胞 小鼠 基因敲除 细胞增殖 氯氟松 骨髓移植 

分 类 号：R457.7[医药卫生—治疗学]