叶用芥菜叶缘裂刻性状的遗传与相关基因表达分析  

Genetic and Related Gene Expression Analysis of Leaf Marginal Traits in Brassica juncea

作　　者：余晨 袁贞贞[2] 沙爱华[1] 万正杰[2] YU Chen;YUAN Zhenzhen;SHA Aihua;WAN Zhengjie(Agricultural College, Yangtze University,Jingzhou,Hubei 434000;College of Horticulture and Forestry,Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology,Ministry of Education,Wuhan,Hubei 430070)

机构地区：[1]长江大学农学院,湖北荆州434000 [2]华中农业大学园艺林学学院园艺植物生物学教育部重点实验室,湖北武汉430070

出　　处：《北方园艺》2019年第6期12-17,共6页Northern Horticulture

基　　金：国家特色蔬菜产业技术体系芥菜品种改良资助项目(CARS-24-A-06);国家重点研发计划资助项目(2017YFD0101803)

摘　　要：以叶缘裂刻和叶缘锯齿的2种芥菜为试材,构建F_2和BC_1分离群体,对群体单株的裂刻性状进行调查,并对相关基因进行qRT-PCR分析,以期明确叶用芥菜叶缘裂刻性状的遗传规律,为芥菜育种提供参考依据。结果表明:芥菜叶缘裂刻性状由1对显性基因控制,将该基因命名为BjLB(Leaf-lobe in Brassica juncea);qRT-PCR分析结果表明,KNAT1基因在叶缘裂刻芥菜中的表达量高于叶缘锯齿芥菜的表达量,与预期结果一致,其它基因在2个亲本中表达差异不明显,推测KNAT1基因有可能是候选基因。Two Brassica juncea mustard materials with leaf margin and leaf margin serration were used as materials,the F2 and BC1 segregating populations were constructed,the splicing traits of individual plants were investigated and qRT-PCR analysis of related genes was performed in order to clarify the inheritance of leaf margin traits of leaf mustard and provide theoretical basis for Brassica juncea breeding.The results showed that the leaf margin of the mustard leaf was controlled by a pair of dominant genes,and the gene was named BjLB(Leaf-lobe in Brassica juncea);qRT-PCR analysis showed that the expression level of KNAT1 gene in the leaf margin was higher than that of the leaf margin,and the other genes difference expressed in the two parents were not obvious,and it was speculated that the KNAT1 gene might be a candidate gene.

关 键 词：芥菜 叶缘裂刻 遗传分析 基因表达 

分 类 号：S637.203.6[农业科学—蔬菜学]