补肾活血中药对慢性心力衰竭大鼠Klotho蛋白及心肌细胞形态的影响  

Effects of Kidney-tonifying and Blood-activating Herbs on Klotho Protein and Myocardial Structure in Chronic Heart Failure Rats

作　　者：孔繁达 张艳[2] 朱爱松[1] 张洋[3] 康伊[4] 张宇[1] KONG Fanda;ZHANG Yan;ZHU Aisong;ZHANG Yang;KANG Yi;ZHANG Yu(Liaoning University of Traditional Chinese Medicine, Shenyang 110847 , Liaoning, China;The First Affilated Hospital of Liaoning University of TCM , Shenyang 110032 , Liaoning, China;The Second Affiliated Hospital of Dalian Medical University, Dalian 116027 , Liaoning, China;The First Affiliated Hospital of China Medical University,Shenyang 110001 ,Liaoning,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032 [3]大连医科大学附属第二医院,辽宁大连116027 [4]中国医科大学附属第一医院,辽宁沈阳110001

出　　处：《中华中医药学刊》2019年第3期619-623,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81774157)

摘　　要：目的:观察补肾活血中药对慢性心力衰竭(CHF)大鼠心功能、心肾组织Klotho蛋白、心肌细胞形态的影响。方法:选取12周龄雄性SD大鼠60只,随机分为4组:假手术组、模型组、赖诺普利对照组(0.25 mg·kg^(-1)·d^(-1))、补肾活血中药组(5.4 g·kg^(-1)·d^(-1))。通过结扎大鼠冠状动脉左前降支配合减食、力竭式游泳造成慢性心衰模型,造模成功后连续灌胃4周,超声检测并计算大鼠左室收缩末期内径(LVSD)左室舒张末期内径(LVDD)左室射血分数(EF)左室短轴缩短率(FS),蛋白质印迹法(Western Blot)测定大鼠心、肾组织中Klotho蛋白表达水平,实时定量聚合酶链式反应法(Real-time PCR)检测大鼠心、肾组织中Klotho mRNA表达水平,电镜下观察心肌细胞形态学变化。结果:根据EF值判定造模成功,经治疗后,与模型组比较赖诺普利对照组、补肾活血中药组大鼠LVSD、LVDD、EF、FS明显改善,差异有统计学意义(P<0.05);与模型组比较,赖诺普利对照组、补肾活血中药组大鼠心、肾组织中Klotho mRNA及Klotho蛋白表达均升高,差异有统计学意义(P<0.05)。结论:补肾活血中药可上调Klotho蛋白的表达,改善慢性心衰大鼠心肌细胞形态,改善大鼠心功能,延缓心室重构,有效治疗慢性心衰。Objective: To observe the effect of of kidney-tonifying and blood-activating herbs on cardiac function, Klotho protein and cardiomyocyte morphology in chronic heart failure rats. Method: Sixty male SD rats were randomly divided into 4 groups: sham operation group, model group, kidney-tonifying and blood -activating herbs group with dose of 5. 4 g·kg^-1· d^-1 , and Lisinopril control group with dose of 0. 25 mg· kg^-1·d^-1. Chronic heart failure model was established by ligaturing anterior descending coronary artery and exhausted swimming. After 4 weeks of continuous administration ,the ultrasound testing was used to detect LVSD, LVDD, EF and FS of rats. Western Blot was used to detect the expression level of Klotho in heart and kidney tissue of rats. Real - time PCR was used to detect the expression level of Klotho mRNA in heart and kidney tissue of rats. Electron microscope was used to observe the myocardial cell morphological changes. Results: The model was successful according to the EF value. After treatment, compared with model group, LVSD, LVDD, EF and FS of rats in Lisinopril group and kidney-tonifying and blood - activating herbs group were significantly improved and the difference was statistically significant ( P < 0. 05 ). Compared with the model group, the expressions of Klotho mRNA and Klotho protein in the heart and kidney tissues of the Lisinopril control group and the kid ney - tonifying and blood -activating herbs group were significantly increased and the difference was statistically significant ( P < 0. 05). Conclusion: Kidney - tonifying and blood - activating herbs can up -regulate the expression of Klotho protein , improve myocardial cell morphology in rats with chronic heart failure, improve cardiac function, delay ventricular remodeling and treat chronic heart failure effectively.

关 键 词：补肾活血 心力衰竭 KLOTHO 细胞形态 

分 类 号：R285.5[医药卫生—中药学]