应用磁共振T_2弛豫自旋标记技术研究多巴胺D4受体-616位点单核苷酸多态性对原发性夜间遗尿症患儿脑氧代谢的影响  

Effect of DRD4 receptor-616 C/G polymorphism on cerebral oxygen metabolism in pediatric primary nocturnal enuresis patients, an MR study using TRUST technique

作　　者：肖珊珊 黄明珠[1] 张旭[1] 侯阳[1] 于兵[1] XIAO Shan-shan;HUANG Ming-zhu;ZHANG Xu;HOU Yang;YU Bing(Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China)

机构地区：[1]中国医科大学附属盛京医院放射科,辽宁沈阳110004

出　　处：《中国临床医学影像杂志》2019年第3期158-161,共4页Journal of China Clinic Medical Imaging

基　　金：国家重点研发计划(项目基金号2016YFC0107100;2016YFC0106804);国家自然科学基金资助项目(项目基金号81541058;81301204)

摘　　要：目的:应用T_2弛豫自旋标记(TRUST)技术,探讨多巴胺D4受体基因(DRD4)616位点(rs747302)单核苷酸多态性(Single nucleotide polymorphism,SNP)对原发性夜间遗尿症(Primary nocturnal enuresis,PNE)患儿脑氧代谢的影响。方法:本研究为前瞻性研究,通过DRD4-616位点基因型检测及TRUST扫描,获取2016年2月—2017年12月56例PNE患儿及58例正常对照组儿童的脑氧代谢数据及基因型数据。以年龄、性别作为协变量,以分组(PNE/对照)及基因型(C/G)作为可能的影响因素,针对脑氧摄取率(Oxygen extraction fraction,OEF)以及脑氧代谢率(Cerebral metabolic rate of oxygen,CMRO2)进行析因设计的协方差分析。结果:OEF以及CMRO2分组×基因型交互效应显著(F_(1,110)=6.513,P=0.012 1;F_(1,110)=6.888,P<0.001;F_(1,110)=13.01,P<0.001),Post-Hoc检验发现结果携带C等位基因的PNE患儿OEF以及CMRO2值显著低于G纯合子PNE患儿(t=-5.976,Padjusted<0.001;t=-5.594,Padjusted<0.001);Yν显著高于G纯合子PNE患儿(t=5.863,Padjusted<0.001)。结论 :DRD4-616 SNP对于PNE患儿OEF及CMRO2造成了影响,这可能与PNE的发病有关。Objective: To determine whether the 616 C/G single nucleotide polymorphism(SNP) in the dopamine D4 receptor(DRD4) promoter rs747302 was associated with abnormal whole-brain cerebral metabolic rate of oxygen(CMRO2), cerebral blood flow(CBF) and oxygen extraction fraction(OEF) in children that suffer from primary nocturnal enuresis(PNE) using T2-relaxation-under-spin-tagging(TRUST) methodology. Methods: Genomic and TRUST imaging data were obtained from 56 PNE pediatric patients and 58 healthy controls. SNP of rs747302 was genotyped. TRUST imaging data were collected on a 3.0-Tesla MR scanner, OEF and CMRO2 data were calculated using an in-house package. OEF and CMRO2 were compared between different group(PNE/control) and genotype(C/G) using two-way ANCOVA analysis. Results: Group-genotype interactions were found in OEF and CMRO2, post-hoc testing demonstrated that the C-allele carriers in the PNE group had a significantly increased OEF and CMRO2. Conclusion: Among PNE children, C-allele carriers of rs747302 are associated with abnormal OEF and CMRO2.

关 键 词：遗尿 受体 多巴胺 儿童 磁共振成像 

分 类 号：R725.8[医药卫生—儿科] R445.2[医药卫生—临床医学]