北豆根中化学成分及其抗缺氧活性研究  

Chemical constituents from rhizome of Menispermum dauricum and their anti-hypoxic activities

作　　者：邵佳[1,2] 师超峰 魏金霞 李雅潇 郭兴杰[3] SHAO Jia;SHI Chao-feng;WEI Jin-xia;LI Ya-xiao;GUO Xing-jie(Department of Pharmacy, Tianjin First Central Hospital, Tianjin 300192, China;Department of Health Service, Logistics College of Chinese People's Armed Police Forces, Tianjin 300309, China;School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China)

机构地区：[1]天津市第一中心医院药学部,天津300192 [2]武警后勤学院卫生勤务系,天津300309 [3]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中国中药杂志》2019年第4期723-729,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81703690;81803356);武警后勤学院博士启动金项目(WHB201711);武警后勤学院科研基金面上项目(WHJ201717);天津市第一中心医院科技基金项目(院CM201806)

摘　　要：采用制备薄层色谱、硅胶柱色谱、ODS柱色谱、D101大孔树脂柱色谱、Sephadex LH-20凝胶柱色谱等色谱分离方法,对北豆根的75%乙醇提取物进行分离纯化,根据化合物的理化性质和波谱数据进行结构鉴定。从北豆根的75%乙醇提取物中分离得到15个化合物,分别鉴定为N-methylcorydaldine(1)、白蓬草福林(2)、千金藤啶碱(3)、阿克吐明(4)、蝙蝠葛苏林碱(5)、阿克吐米定(6)、蝙蝠葛新苛林碱(7)、蝙蝠葛辛碱(8)、6-O-去甲基蝙蝠葛波酚碱(9)、蝙蝠葛定碱(10)、蝙蝠葛糖苷(11)、刺五加苷D(12)、马兜铃内酯(13)、马兜铃酸萜酯Ⅰ(14)和马兜铃酸(15),化合物12～15为首次从蝙蝠葛属植物中分离得到。利用MTT法对所分得量较大的6种生物碱进行了抗缺氧活性研究,结果表明6种生物碱均具有较显著的抗缺氧活性。To study the chemical constituents from the rhizome of Menispermum dauricum, fifteen compounds,N-methylcorydaldine (1), thalifoline (2), stepholidine (3), acutumine (4), daurisoline ( 5), acutumidine ( 6), dauricicoline (7), bianfugecine (8), 6-O-demethylmenisporphine (9), bianfugedine (10), dauricoside (11), eleutheroside D (12), aristolactone (13), aristoloterpenate Ⅰ(14) and aristolochic acid (15) were isolated from 75% ethanol extract of Menispermi Rhizoma by using thin layer chromatography and column chromatography methods. Their structures were identified based on their physicochemical properties and spectral data. Among them, compounds 12-15 were obtained from the genus Menispermum for the first time. Six alkaloids with higher contents were subjected to evaluate the anti-hypoxic activities by using MTT method. As a result, six alkaloids exhibited significant anti-hypoxia activities.

关 键 词：北豆根 化学成分 生物碱 抗缺氧活性 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]