风湿祛痛胶囊对TNF-α诱导的人类风湿关节炎成纤维样滑膜细胞功能的影响  

Effect of Fengshi Qutong Capsule on Function of Human Synovial Cells in Rheumatoid Arthritis Induced by TNF-α

作　　者：何莲花[1,2] 李逸群 王靖霞 孙丛丛 刘春芳[1] 荆宇[3] 苗艳东 林娜 HE Lian-hua;LI Yi-qun;WANG Jing-xia;SUN Cong-cong;LIU Chun-fang;JING Yu;MIAO Yan-dong;LIN Na(Institute of Chinese Materia Medica,China Academy of Chinese Medicine,Beijing 100700,China;Peking University Shenzhen Hospital,Shenzhen 518036,China;Tonghua Golden-horse Group,Beijing 100028,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]北京大学深圳医院,广东深圳518036 [3]通化金马药业集团股份有限公司,北京100028

出　　处：《中国实验方剂学杂志》2019年第7期116-121,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院中药研究所技术研发项目(20171011)

摘　　要：目的:研究风湿祛痛胶囊(FSQTC)对肿瘤坏死因子-α(TNF-α)诱导的人类风湿关节炎(RA)成纤维样滑膜细胞增殖、迁移、黏附、侵袭和分泌功能的影响。方法:TNF-α(20μg·L^(-1))体外诱导RA患者成纤维样滑膜细胞(MH7A),加入不同浓度FSQTC(0. 02,0. 1,0. 5μg·L^(-1))作用后,分别采用噻唑蓝(MTT)比色法,转移小室(transwell)迁移、黏附及侵袭实验检测MH7A细胞的增殖活性、迁移、黏附及侵袭能力,酶联免疫吸附测定(ELISA)检测细胞上清中白细胞介素(IL)-1β及血管内皮细胞生长因子(VEGF)的含量。结果:与空白组比较,TNF-α(20μg·L^(-1))能显著增加MH7A细胞的增殖、迁移、黏附、侵袭能力及分泌IL^(-1)β和VEGF的含量(P <0. 01);与TNF-α组比较,FSQTC(0. 02,0. 1,0. 5μg·L^(-1))作用24 h对TNF-α诱导的MH7A细胞增殖活性没有明显影响,作用48 h能浓度依赖地降低TNF-α诱导增加的MH7A细胞增殖活性(P <0. 05,P <0. 01),作用24 h还能显著降低TNF-α诱导升高的MH7A细胞迁移、黏附、侵袭能力及分泌IL^(-1)β和VEGF的含量(P <0. 05,P <0. 01)。结论:FSQTC可降低MH7A细胞增殖、迁移、黏附、侵袭及分泌IL^(-1)β和VEGF的能力。Objective:To study the effects of Fengshi Qutong capsule(FSQTC)on proliferation,migration,adhesion,invasion and secretion of human synovial cells in rheumatoid arthritis(RA)induced by tumor necrosis factor-α(TNF-α)and explore its mechanism.Method:Human synovial cells(MH7A)in RA patients were induced in vitro by using TNF-α(20 μg·L^-1).After treatment with different concentrations of FSQTC(0.02,0.1,0.5 μg·L^-1),MTT colorimetric assay,transwell migration,adhesion and invasion tests were used to detect the proliferation,migration,adhesion and invasion of the MH7A,respectively.The expression levels of vascular endothelial growth factor(VEGF)and interleukin-1β(IL-1β)in MH7A supernatant were detected by enzyme linked immunosorbent assay(ELISA).Result:As compared with blank control group,TNF-α(20 μg·L^-1)significantly increased the proliferation,migration,adhesion,invasion and secretion of IL-1β and VEGF of MH7A cells(P<0.01).FSQTC(0.02,0.1,0.5 μg·L^-1)had no significant effect on proliferation of TNF-α-induced MH7A cells after treatment for 24 hours.After 48 hours of treatment,proliferation of MH7A cells induced by TNF-α was decreased in a concentration-dependent manner(P<0.05,P<0.01).Within 24 hours,the migration,adhesion,invasion,invasion,and secretion of IL-1 and VEGF in MH7A cells were also decreased significantly(P<0.05,P<0.01).Conclusion:FSQTC can inhibit the proliferation,migration,adhesion,invasion and secretion of IL-1β and VEGF in MH7A cells.

关 键 词：风湿祛痛胶囊 成纤维样滑膜细胞(MH7A) 增殖 迁移 侵袭 黏附 

分 类 号：R289[医药卫生—方剂学] R593.22[医药卫生—中药学]