高+Gz暴露对种植体周围骨组织核因子КB受体活化因子配体及骨保护素mRNA表达的影响  

Influence of high +Gz environment on receptor activator of nuclear factor κB ligand and osteoprotegerin mRNA expression in the peri-implant bone tissue

作　　者：朱晓茹 邓天政 逄建梁 刘冰 柯杰 Zhu Xiaoru;Deng Tianzheng;Pang Jianliang;Liu Bing;Ke Jie(Air Force Medicine Center,PLA,Beijing 100142,China)

机构地区：[1]空军特色医学中心口腔科,北京100142

出　　处：《中华航空航天医学杂志》2018年第3期231-235,F0003,共6页Chinese Journal of Aerospace Medicine

基　　金：中国博士后科学基金面上资助项目(2O16M592971).

摘　　要：目的采用动物模型观察高正加速度环境对种植体周围骨组织核因子κB受体活化因子配体(receptor activator of nuclear factor κB ligand,RANKL)及骨保护素(osteoprotegerin,OPG)信使核糖核酸(messengerRNA,mRNA)表达的影响。方法按随机数字表将30只新西兰白兔随机分为3周对照组、3周实验组、5周对照组、5周实验组、12周对照组、12周实验组6组,每组各5只。拔除其双侧下颌切牙后即刻植入种植体各1颗。术后1周开始每周将实验组动物高正加速度(+Gz)暴露3次(周一、周三、周五);暴露方案为加速度增长率1G/s、峰值4~9G、峰值持续时间10~45s、间隔1min的一组梯形加速度曲线。对照组不进行+Gz暴露,常规饲养。在术后3周(+Gz暴露2周)、5周(+Gz暴露4周)、12周(+Gz暴露4周+常规饲养7周)分别各处死1组实验组和1组对照组白兔,取种植体周围骨质标本,釆用实时荧光定量聚合酶链式反应(real-time polymerase chain reaction,RT-PCR)检测RANKL及OPG的mRNA表达情况。结果实验组和对照组RANKL及OPG的mRNA表达均在术后3周时最高,然后逐渐降低。术后3周时,实验组RANKL的mRNA表达以及RANKL/OPG比值明显高于对照组(P<0,05);术后5周,实验组OPG的mRNA表达明显低于对照组,而RANKL的mRNA表达以及RANKL/OPG比值仍明显高于对照组(P<0,05);12周时两组各指标表达没有明显差异。结论高+Gz暴露能够促进RANKL的表达,提高RANKL/OPG比值,促进骨吸收,不利于种植体骨结合。Objective To investigate the influence of high+Gz environment on receptor activator of nuclear factor κB ligand(RANKL ) and osteoprotegerin(OPG) messenger RNA(mRNA) expression in the peri-implant bone tissue by an animal model. Methods Thirty New Zealand white rabbits were randomly and averagely divided into 3 control groups and 3 experimental groups corresponding to the investigation for 3 weeks,5 weeks and 12 weeks respectively. Rabbits' mandible incisors of both sides were extracted and implanted with 1 implant in each socket immediately. After 1 week of rest,the experimental rabbits were exposed to high+Gz environment 3 times a week(Monday,Wednesday,Friday),The exposure scheme choose the+Gz profile with 4~9 G for 10.45 s with 1 G/s onset rate and with the intervals of 1 min. The rabbits of control groups were not exposed to+Gz environment and normally fed. One control group and one experimental group rabbits were respectively sacrificed at the 3rd week(+Gz exposure for 2 weeks),5th week(+Gz exposure for 4 weeks) and 12th week(+GZ exposure for 4 weeks and normal feeding for 7 weeks) after the implanting surgery. The specimens were obtained to find the mRNA expression of RANKL and OPG by real-time polymerase chain reaction(RT-PCR) examination. Results The mRNA expression of RANKL and OPG were the highest 3 weeks after the surgery in both groups,and then decreased gradually. The RANKL mRNA expression and the RANKL/OPG ratio of the experimental group were significantly higher than those of the control group 3 weeks after the surgery(P<0.05). Five weeks after the surgery,the OPG mRNA expression of the experimental group was significantly lower than that of the control group(P<0.05),but the RANKL mRNA expression and RANKL/OPG ratio were still higher than that of control group(P<0.05). Twelve weeks after the surgery,the differences on RANKL and OPG mRNA expressions was not obvious between two groups. Conclusions High+Gz exposure could increase the expression of RANKL,raise RANKL/OPG ratio and promote bone resorptio

关 键 词：加速度 兔 牙种植体 核因子ΚB受体活化因子 骨保护素 

分 类 号：R852.21[医药卫生—航空、航天与航海医学] R782.1[医药卫生—临床医学]