注射用双黄连(冻干)的UPLC指纹图谱研究及质量评价  

UPLC fingerprint study and quality evaluation of Shuang-Huang-Lian lyophilized powder

作　　者：冯五文 王晶 张世洋 唐飞 盛永成 敖慧[1] 彭成[1] FENG Wu-wen;WANG Jing;ZHANG Shi-yang;TANG Fei;SHENG Yong-cheng;AO Hui;PENG Cheng(School of Pharmacy,Chengdu University of TraditionalChinese Medicine,Key Laboratory of Standardization for Chinese Herbal Medicine,Ministiy of Education,National Key LaboratoryBreeding Base of Systematic Research,Development and Utilization of Chinese Medicine Resources,Chengdu 611137,Sichuan)

机构地区：[1]成都中医药大学药学院,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,中药材标准化教育部重点实验室,四川成都611137

出　　处：《中药与临床》2018年第6期31-33,8,共4页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金人才培养项目;成都中医药大学中药基础基地科研训练及科研能力提高项目(J1310034)

摘　　要：目的:建立注射用双黄连(冻干)的UPLC指纹图谱并用于评价不同批次双黄连冻干粉。方法:采用ACQUITY UPLC RHSS T3色谱柱;以乙腈与0.2%甲酸水为流动相;柱温室温;进样量5μL;流速0.2 mL﹒min-1,梯度洗脱;检测波长350 nm。收集并制备双黄连冻干粉不同样品,采用相似度分析及聚类分析区分不同的双黄连冻干粉样品。结果:标定了正常双黄连冻干粉样品的26个共有峰,并结合对照品比对确定了其中11个成分。通过聚类分析,所有特殊双黄连冻干粉样品均可以与正常样品区分。结论:所建立的UPLC指纹图谱具有快速、高效的特点,可用于双黄连冻干粉的质量检测。Objective:To establish UPLC fingerprint for Shuang-Huang-Lian lyophilized powder(SHL)and to evaluate the quality of different SHL samples.Method:The chromatographic fingerprints were obtained with ACQUITY UPLC HSS T3 column.Gradient elution with acetonitrile and aqueous formic acid was carried at the flow rate of 0.2 mL·min^-1.The detection wavelength was set at 350 nm.The injection volume was 5μL.Different SHL samples were collected,and similarity analysis and clustering analysis were performed to differentiate SHL samples.Result:26 common peaks in normal SHL samples were marked and 11 constituents were identified by reference standards.By clustering analysis,normal samples were differentiated.Conclusion:The established UPLC method with fast,efficient advantage can be used to detect the quality of SHL sample.

关 键 词：注射用双黄连(冻干) 指纹图谱 质量评价 超高效液相 

分 类 号：R285.5[医药卫生—中药学]