FGR的胎盘组织印记基因PEG10DNA甲基化水平及其意义  

Methylation state of imprinted gene PEG10 DNA in placental tissue of patients with FGR

作　　者：梁小妍[1] 金玉珍[1] 马丽[1] 陈雄[1] LIANG Xiaoyan;JIN Yuzhen;MA Li;CHEN Xiong(Department of Obstetrics and Gynecology,Baoshan Branch of Shanghai First People's Hospital,Shanghai 200940,China)

机构地区：[1]上海市第一人民医院宝山分院妇产科,上海200940

出　　处：《中国妇幼健康研究》2019年第3期307-311,共5页Chinese Journal of Woman and Child Health Research

基　　金：上海市科委医学引导资助项目(项目编号:15411971100);上海市卫生和计划委员会青年资助项目(项目编号:20144Y0083)

摘　　要：目的通过检测父方表达印记基因PEG10 DNA在胎儿生长受限(FGR)患者胎盘组织甲基化水平,探索FGR是否与其胎盘中印记基因的甲基化程度相关。方法采用前瞻性研究,收集2014年1月至2017年1月在上海市第一人民医院宝山分院妇产科住院分娩的FGR者56例(研究组)和同期正常单胎晚孕分娩者56例(对照组),采用亚硫酸氢盐测序法(BSP)检测研究组和对照组胎盘组织的PEG10 DNA甲基化水平,分析其甲基化水平与FGR的临床病理关系。结果 FGR胎盘组织印记基因PEG10表现为异常甲基化水平,与对照组相比,研究组胎盘组织印记基因PEG10 DNA甲基化水平在CPG岛1无明显差异,而在CPG岛2及CPG岛3明显高于对照组(t值分别为94.97,7.16,均P<0.05),尤其以CPG岛2最为明显(研究组表达为0.544±0.027,对照组表达为0.152±0.015)。结论胎盘组织父方表达的印记基因的DNA甲基化水平可能是导致FGR发病的原因之一。Objective To investigate the relationship between methylation state of imprinted gene (PEG10)paternally expressed gene 10 DNA in patient’s placental tissues with fetal growth restriction (FGR) by detecting the methylation level of father’s PEG 10 DNA in placental tissues.Methods Prospective study was conducted on the clinical date of 56 women with FGR (case group) delivering in Baoshan Branch of Shanghai First People’s Hospital during January 2014 to January 2017 as well as 56 normal pregnant women with single birth (control group).Hydrogen sulfite sequencing (BSP) was used to evaluate DNA methylation levels of PEG10 in placental tissues,and the clinicpathological relationship between methylation level and FGR was analyzed.Results The characteristic of imprinted genes PEG10 was abnormal methylation of FGR in placental tissue.Compared with the control group,there was no significant difference in the level of DNA methylation of the placenta imprinting gene PEG10 in CPG Island 1,whereas the level in CPG Island 2 and CPG Island 3 was significantly higher in the case group ( CPG Island 2 t =94.97,CPG Island 3 t =7.16,both P <0.05).CPG Island 2 was most obvious (0.544±0.027 in case group and 0.152 0.015 in the control group).Conclusion Methylation’s level of the imprinted gene expressed in father in placental tissue might be one of the causes of FGR.

关 键 词：胎儿生长受限 印记基因PEG10 甲基化 亚硫酸氢盐测序法 胎盘组织 

分 类 号：R714.5[医药卫生—妇产科学]