马钱苷调节UVB诱导的表皮黑素单位黑素生成的实验研究  

Experimental Study on Loganin Regulating the Generation of Melanin in UVB-induced Epidermal Melanin Unit

作　　者：李伟[1] 王加志[1] 曲岩[1] 王兴焱[1] 陈景华[1] 党文军[1] LI Wei;WANG Jiazhi;QU Yan;WANG Xingyan;CHEN Jinghua;DANG Wenjun(Jiamusi College,Heilongjiang University of Chinese Medicine,Jiamusi 154007,China)

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《西部中医药》2019年第2期13-16,共4页Western Journal of Traditional Chinese Medicine

基　　金：黑龙江省自然科学基金面上项目(编号H2015039);黑龙江省中医药管理局中青年科技攻关项目(编号ZQG-018);黑龙江中医药大学校基金(编号201317);黑龙江中医药大学佳木斯学院院基金(编号JYKY201504)

摘　　要：目的:探讨不同浓度马钱苷对中波紫外线(UVB)诱导该模型中黑素生成及其作用的影响。方法:常规体外共培养人黑色素瘤A375、人皮肤角质形成细胞(HaCaT),以UVB20 m J/cm^2照射后加含马钱苷的培养液作用24h,四甲基偶氮唑蓝(MTT)比色法测定细胞增殖;采用多巴氧化法研究酪氨酸酶活性的变化;比色法测定黑素含量,免疫印迹法测定P38通路蛋白表达。结果:马钱苷0.01μm/L可抑制UVB诱导的表皮黑素单位中酪氨酸酶活性增高,减少黑素生成,其机制可能是通过抑制P38蛋白表达减弱信号通路作用,抑制酪氨酸酶活性而实现的。结论:高浓度马钱苷可明显抑制P38MAPK通路,进而抑制酪氨酸酶活性和黑素生成。Objective:To discuss the effects of different concentrations of loganin on the generation of melanin in UVB-induced epidermal melanin unit and the mechanism.Methods:A375 and Hacat cells were co-cultured in vitro as routine,after radiated with UVB 20 mJ/cm^2 and adding nutrient solution containing loganin for 24 hours,cellular proliferation was detected using MTT chromatometry;the activity changes of tyrosinase were studied using Dopa oxidation method;the contents of melanin were detected using chromatometry,and the expressions of P38 pathway protein were measured using immunoblotting.Results:Loganin in the concentrations of 0.01μm/L could inhibit the activity increase of tyrosinase in UVB-induced epidermal melanin unit and reduce the generation of melanin,and the mechanism might be realized by inhibiting the expressions of P38 protein,reducing signaling pathway and inhibiting the activity of tyrosinase.Conclusion:High concentrations of loganin could obviously inhibit P38MAPK pathway,therefore to inhibit the activity of tyrosinase and the generation of melanin.

关 键 词：马钱苷 酪氨酸酶 表皮黑素单位 P38MAPK 

分 类 号：R285[医药卫生—中药学]