Sequence Analysis and Molecular Markers of a Copialike Retrotransposon Linked to the Color around the Stone(Cs) Locus of the Peach  

桃果肉近核色素copia-like反转录转座子序列分析及分子标记的建立（英文）

作　　者：YAN Xi-guang WANG Zhao-yuan CHANG Rui-feng LIU Guo-jian CHEN Hu XU Jin-tao LI Yong-hong HAN Ji-cheng 闫希光;王召元;常瑞丰;刘国俭;陈湖;徐金涛;李永红;韩继成(河北省农林科学院昌黎果树研究所,河北昌黎066600)

机构地区：[1]Institute of Fruit,Hebei Academy of Agriculture and Forestry Sciences,Changli,066600,PRC

出　　处：《Agricultural Science & Technology》2018年第5期12-23,共12页农业科学与技术（英文版）

基　　金：河北省财政项目(Nos.F18R06001-1,F18R06001-2,F18R19-1002);国家现代桃产业技术体系项目(CARS-30-Z-02);河北省支撑项目(17226341)~~

摘　　要：Retrotransposons are present in all plant genomes and play important roles in genome size,genome structure remodeling,gene function and genome evolution.Me07Em02 marked by sequence-related amplified polymorphism (SRAP) is verified,recovered,sequenced and analyzed,which closely link to the flesh color around the stone.By comparison with the peach genome Peach v1.0,a part of the sequence of copia-like retrotransposons is obtained.With the aid of DANMAN and DNASTAR sequence analysis software,BLASTn alignment is carried out in the peach genome database and GenBank.The whole genome sequence of the copialike retrotransposon in the peach is located at 9 939 764~9 944 771 bp in Scaffold-1 of Peach v1.0,with a total length of 5 008 bp.The LTR on both sides is exactly the same,the length is 444 bp,and the largest ORF box is located at the 487~4 545 bp of the sequence,encoding 1 535 amino acids.The amino acid sequence was submitted to GenBank for Protein BLAST alignment,The results showed that this sequence also has the typical amino acid sequence characteristics of the copia-like retrotransposon.At the same time,the method of peach retrotransposon labeling is preliminarily established,and the genetic diversity of the peach is analyzed.通过对与桃果实近核色素紧密连锁的SRAP标记Me07Em02扩增得到的片段进行了验证、回收、测序和分析,并登录桃基因组Peach v1.0进行比对,获得部分copia-like反转录转座子的部分序列,应用DANMAN和DNASTAR序列分析软件辅助,在桃基因组数据库和GenBank中进行BLASTn比对获得了桃copia-like反转录转座子的全基因组序列,位于Peach v1.0基因组的Scaffold-1的9 939 764～9 944 771 bp位置,全长5 008 bp,其左右两边的长末端重复序列(LTR)完全一致,长度为444 bp,其最大开放阅读框(ORF)位于该序列的487～4 545bp,编码1 535个氨基酸,将该氨基酸序列提交到GenBank中进行Protein BLAST比对,结果显示该序列也具有典型的copia-like反转录转座子的氨基酸序列特征。同时初步建立了桃反转录转座子标记方法,并对桃的遗传多样性进行了分析。

关 键 词：PEACH Copia-like retrotransposon Sequence-related amplified polymorphism (SRAP) Genetic diversity 

分 类 号：S662.1[农业科学—果树学]