Construction and Application of a High-throughput Method for Identifying Maize Resistance to Aspergillus flavus in the Field  

玉米抗黄曲霉田间高通量鉴定方法构建及应用（英文）

作　　者：QI Yao-cheng DING Jian-zhou ZUO Xiao-long ZHANG Wei QIAN Yi-liang REN Si-hai WANG Jun GUO Jin RUAN Long 齐耀程;丁舰舟;左晓龙;张玮;钱益亮;任四海;王俊;郭进;阮龙(安徽省农业科学院烟草研究所,安徽合肥230031)

机构地区：[1]Tobacco Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,PRC

出　　处：《Agricultural Science & Technology》2018年第5期43-49,共7页农业科学与技术（英文版）

基　　金：安徽省科技攻关计划项目(1604a0702021);安徽省农业科学院院长青年创新基金项目(17B0916);安徽省农业科学院玉米种质创制利用与创新团队项目(18C0919);安徽省重点研究与开发计划项目(1704f0704069);安徽省自然科学基金项目(1808085QC87)~~

摘　　要：In order to establish a fast and effective high-throughput method for identifying maize resistance to Aspergillus flavus infection in the field,5 conidial concentrations and 3 inoculation methods were compared and optimized. A correlation model between disease levels and aflatoxin B1 accumulation was established. The results showed that inoculating maize inbred lines with the optimal conidial concentration of 1.0×10^6 c/mL could accurately show the different resistance levels of maize to A.flavus infection,and the spiral quantitative inoculation technique could effectively avoid the defect of calculation error caused by commonly used methods. The positive correlation model of resistance level and aflatoxin B1 accumulation could effectively predict the capability of maize being resistant to A.flavus. 8 resistant and 9 medium resistant maize inbred lines of the RIL population were identified.The spiral quantitative identification method realized the high-throughput identification of maize inbred lines resistant to A.flavus infection in the field,and the results have shown high accuracy,stability and repeatability.为构建快速有效的玉米抗黄曲霉菌的田间高通量鉴定方法,本研究分别对5个递增的黄曲霉菌孢子浓度和3种接种方式进行对比优化,建立病情级别与黄曲霉毒素B1积累量的相关模型。结果表明,不同抗性玉米自交系在孢子浓度为1.0×10~6 c/mL浓度的抗性能力最准确,使用螺旋定量接种法避免造成发病菌圈重叠等缺点,利用病情级别与黄曲霉毒素B1积累量的正相关模型有效预测玉米自交系对黄曲霉的抗性能力。螺旋定量鉴定出自育重组自交系后代群体中抗性自交系8个,中抗性自交系9个,实现了抗黄曲霉菌玉米自交系在田间环境的高通量鉴定,结果准确性高,有较好的稳定性和重复性。

关 键 词：MAIZE Aspergillus flavus Inoculation method Disease level Resistance evaluation 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]