鲫葡萄糖调节蛋白78基因部分序列的克隆、鉴定及功能预测  

Cloning,Identification,and Functional Prediction of Partial Gene Sequence of Glucose Regulated Protein 78 in Carassius auratus

作　　者：刘小花 赵元莙 鄢彦杰 张金叶 LIU Xiaohua;ZHAO Yuanjun;YAN Yanjie;ZHANG Jinye(Chongqing Key Laboratory of Animal Biology,College of Life Sciences,Chongqing Normal University,Chongqing 101331,China)

机构地区：[1]重庆师范大学生命科学学院动物生物学重庆市重点实验室,重庆401331

出　　处：《重庆师范大学学报（自然科学版）》2019年第2期18-23,F0002,共7页Journal of Chongqing Normal University:Natural Science

基　　金：国家自然科学基金(No.31672280;No.31471980;No.31601845);重庆市基础与前沿研究项目(No.cstc2014jcyjA00006);重庆市教育委员会科学技术研究项目(No.KJ1400515)

摘　　要：【目的】分析和预测鲫(Carassius auratus)葡萄糖调节蛋白78(Glucose regulated protein 78,GRP78)的功能,为研究GRP78在鱼类受细胞外刺激过程中的作用机制奠定基础。【方法】通过分子克隆技术获得鲫GRP78基因的部分序列,并利用生物信息学方法分析了该基因片段编码的蛋白质序列的二级结构、亲水性、可塑性、表面可及性和抗原表位。【结果】获得了长度为617bp的鲫GRP78基因片段序列,共编码113个氨基酸。这些氨基酸所构成序列的相对分子质量约为1.3kDa,等电点为5.82,无信号肽;可塑性区域位于第7～10位、第18～21位、第28～42位、第51～62位、第68～78位和第94～109位氨基酸残基;表面可及性区域位于第29～30位、第38～43位、第50～65位、第68～79位、第82～86位、第97～99位、第102～104位和第106～111位氨基酸残基;有1个B细胞抗原表位区域,位于第28～45位氨基酸残基。【结论】所获的鲫GRP78序列中含有ATPase保守结构域区段,该区段定位于内质网,具有较强亲水性。[Purposes]To analyze and predict the function of glucose regulated protein 78(GRP78)in Carassius auratus and provide a foundatio n for studying the mechanism of GRP78 during the process of extracellular stress in fish.[Methods]A partial seque nee of GRP78 gene in C.auratus was cloned,whose physicochemical property including secondary structures,hydrophilicity,flexibility,surface probability,antigen epitope,was analyzed by bioinformatics methods.[Findings]The GRP78 gene fragment sequence with long 617 bp was obtained.The sequence encodes a total of 113 amino acids with molecular weight about 1.3 kDa and pl 5.82.None signal peptide was existed.The flexibility was located at 7th^10th,18th.21st,28th.42nd,51st.62nd,68th.78th,and 94th.109th amino acid residues;the surface probability was placed at 29th.30th,38th.43rd,50th.65th,68th.79th,82nd.86th,97th.99th,102nd^104th,and 106th.111th amino acid residues.Additionally,one B cell epitope was predicted as 28th.45th amino acids residues.[ConclusionsJ ATPase domain was obtained in the auratus GRP78 gene fragmeni with higher hydrophilicity,which is located in ER.

关 键 词：鲫 GRP78基因 生物信息学分析 

分 类 号：Q958.9[生物学—动物学]