miR-23基因家族进化分析及其在猪睾丸组织中的表达变化  

Evolutionary Analysis of miR-23 Gene Family and Its Expression in Pig Testis

作　　者：罗荟 冉茂良[1,2] 翁波 彭馥芝[1,2] 高虎 陈斌 LUO Hui;RAN Maoliang;WENG Bo;PENG Fuzhi;GAO Hu;CHEN Bin(College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;Key Laboratory of Livestock and Poultry Genetic Improvement, Changsha 410128, China)

机构地区：[1]湖南农业大学动物科技学院,长沙410128 [2]畜禽遗传改良湖南省重点实验室,长沙410128

出　　处：《经济动物学报》2019年第1期36-43,共8页Journal of Economic Animal

基　　金：国家现代农业产业技术体系建设专项资金(CARS-36)

摘　　要：运用生物信息学方法在miRBase数据库检索到miR-23基因家族的序列,利用Ensembl数据库信息确定miR-23在基因组的位置,采用MEGA5.0构建miR-23系统进化树,再利用RT-q PCR技术定量检测其在沙子岭猪睾丸组织中7个不同发育时期(D1、D30、D60、D90、D120、D150、D180)的表达变化。结果表明:在37种物种中共搜索到82条序列,且各序列具有高度保守性,大部分位于基因间隔区。进化分析表明:各物种miR-23基因家族可能以不同的方式和速度进化而来。RT-q PCR检测结果显示,miR-23基因家族在猪睾丸组织中不同发育时期表现出相同趋势的表达规律,即出生后其表达水平有所降低,到性成熟期又呈现升高趋势。本研究对miR-23基因家族进行系统进化分析并检测其在沙子岭猪睾丸组织中表达变化规律,为进一步探明miR-23基因家族的功能及作用机制奠定基础。The sequences of miR-23 gene family were retrieved from the miRBase database using bioinformatics methods. The position of miR-23 in the genome was determined using the Ensembl database. The miR-23 phylogenetic tree was constructed using MEGA 5. 0,and then quantified using RT-qPCR. The expression of the gene in seven different developmental stages( at days 1,30,60,90,120,150 and 180) of the pig testis in Shaziling was detected. The results showed that 82 sequences were found in 37 species,and the sequences were highly conserved. Most of them were located in the intergenic region. Evolutionary analysis showed that the miR-23 gene family of various species might have evolved in different ways and at different speeds. The results of RT-qPCR showed that the miR-23 gene family showed the same trend of expression at different developmental stages in the pig testis tissues,which the expression level of the miR-23 gene family was decreased after birth and increased again during the sexual maturation period. In this study,the miR-23 gene family was systematically,phylogenetically analyzed and its expression patterns in the testis of pigs in Shaziling were examined,which laid the foundation for further exploration of the functions and mechanisms of the miR-23 gene family.

关 键 词：猪 miR-23a/23b 进化 定量表达 

分 类 号：S828.2[农业科学—畜牧学] Q786[农业科学—畜牧兽医]