单颗粒示踪技术及其在动物病毒入侵机制研究中的应用  

Advance of Single-particle Tracking Technology and Its Applications in Invasion Mechanism of Animal Virus

作　　者：余婉婷[1,2] 王乃东 YU Wanting;WANG Naidong(College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;Hunan Provincial Key laboratory of Protein Engineering in Animal Vaccines, Changsha 410128 , China)

机构地区：[1]湖南农业大学动物医学院,长沙410128 [2]兽用蛋白质工程疫苗湖南省重点实验室,长沙410128

出　　处：《经济动物学报》2019年第1期57-62,共6页Journal of Economic Animal

基　　金：湖南省教育厅重点项目(15A086);湖南省自然科学基金面上项目(2018JJ2177);湖南省研究生科研创新项目(CX2016B285; CX2016B314)

摘　　要：病毒入侵宿主细胞的分子机制十分复杂,是病毒学界的研究热点。单颗粒示踪技术能够直接观察单个病毒入侵细胞的动态行为,有助于解析病毒入侵宿主细胞的分子机制。综述单颗粒示踪技术用于病毒与宿主细胞相互作用的不同过程,有助于动物病毒入侵机制研究和新型抗病毒药物与疫苗的研发。文中主要论述单颗粒示踪技术的发展历程,介绍该技术用于病毒和细胞亚结构的标记方法和进展,以及单颗粒示踪技术在动物病毒入侵机制研究中的应用进展,并总结现阶段单颗粒示踪技术在病毒学发展中的优势和局限性。As a research hotspot in virology,the molecular mechanism of virus invasion into host cells is particularly complex. Single-Particle tracking technology enables us to directly observe the dynamic behavior of one virus invading cells and contributes to analyze the interaction between viruses and host cells. Reviewing the applications of Single-Particle tracking in different processes of the interaction between virus and host cells would contribute to the research of the mechanism of animal virus invasion and the development of new antiviral drugs and vaccines. focuses on The development of Single-Particle tracking technology,the labeling method of virus and cell substructure,and the applications of Single-Particle tracking technology and the mechanism of animal virus invasion were reviewed in the article. The advantages and limitations of the technique were addressed.

关 键 词：单颗粒示踪技术 病毒 细胞亚结构 内吞 荧光标记 

分 类 号：S852.65[农业科学—基础兽医学]