一株鸽Ⅰ型副粘病毒的分离鉴定及F基因遗传进化分析  

Isolation and Identification of One Strain of Pigeon Paramyxovirus Serotype Ⅰ and Phylogenetic Analysis of F Gene

作　　者：李祥[1] 孙静[1] 智敏[1] 杨晓宇 付恬 吕欣孺 马沛然 杨思远[2] 柴洪亮[1] LI Xiang;SUN Jing;ZHI Min;YANG Xiaoyu;FU Tian;LU Xinru;MA Peiran;YANG Siyuan;CHAI Hongliang(College of Wildlife Resources,Northeast Forestry University,Harbin,150040,China;Heilongjiang Vocational College For Nationalities,Harbin,150066,China)

机构地区：[1]东北林业大学野生动物资源学院,哈尔滨150040 [2]黑龙江民族职业学院,哈尔滨150066

出　　处：《野生动物学报》2019年第2期440-444,共5页CHINESE JOURNAL OF WILDLIFE

基　　金：国家自然科学基金(31601873);十三五重点研发专项:迁徙野生动物疫病传播风险(2016YFC1201600);中央高校基本科研业务费(2572017CA19)

摘　　要：东北某动物园鸽群发生异常死亡,采集病死鸽脏器,通过SPF鸡胚进行病毒分离,采用血凝试验和RT-PCR试验,鉴定结果显示尿囊液呈Ⅰ型副粘病毒核酸阳性。通过对F基因进行测序和遗传进化分析,结果表明,分离株BS0904裂解位点基序为112RRQKRF117,符合NDV强毒株的典型分子特征;分离株F基因与疫苗株LaS ota、B1、Clone 30、VG/GA、V-4、Mukteswar及经典强毒株F48E9的核苷酸同源性较低,仅为84. 2%—85. 8%;而与2011—2013年在中国多个地区鸽中所分离毒株核苷酸同源性则高达98. 8%—99. 9%,并且在遗传进化树上位于同一分支,属于ClassⅡ,基因型为Ⅵb-EU/re。该基因型毒株在中国鸽群中有较长时间、较大范围的流行传播,且与疫苗株遗传进化关系较远,说明疫苗株不能对鸽群提供有效的免疫保护,因此为了更好地对鸽群进行Ⅰ型副粘病毒的有效防控,需要针对Ⅵb基因型研发新疫苗。Abnormal death of pigeons was documented in a zoo in northeast China,a pigeon paramyxovirus serotypeⅠ(PPMV-1)isolate was recovered from samples collected from dead pigeons and initial isolation of the virus was performed in specific-pathogen-free(SPF)chicken embryos.The isolate was identified by the hemagglutination(HA)test and RT-PCR.Sequencing and phylogenetic analysis of F gene indicated that the cleavage sites of the BS0904 isolate was 112 RRQKRF 117 motif in accordance with characteristic of velogenic strains.The isolate shared 84.2%-85.8%nucleotide identity with the vaccine strains LaSota,B1,Clone 30,VG/GA,V-4,Mukteswar,and high virulent strain F48E9.But the isolate showed a high level of nucleotide identity(98.8%-99.9%)with isolates obtained from pigeons in regions of China from 2011 to 2013.It also aligned into the same sub-branch in the phylogenetic tree,which belongs to ClassⅡ,genotypeⅥb-EU/re.The genotype strains had spread among pigeons in China for a long time and over a wide geographic range.They exhibited long distance from the vaccine stains,therefore,new vaccines phylogenetically close to the prevalent genotypeⅥb PPMV-1 should be developed to prevent the PPMV-1 infection.

关 键 词：鸽Ⅰ型副粘病毒 F基因 Ⅵb基因型 遗传进化分析 

分 类 号：S852.65[农业科学—基础兽医学]