麻醉剂丁香酚对鳗弧菌抑菌效果初步研究  

Preliminary study of antibacterial effects of fish anesthetic eugenol on Vibrio anguillarum

作　　者：李朝 柯常亮[2] 古小莉[2] 李惠青 黎智广[2] 黄珂[2] 李刘冬[2] 李来好[2] LI Chao;KE Changliang;GU Xiaoli;LI Huiqing;LI Zhiguang;HUANG Ke;LI Liudong;LI Laihao(Zhejiang Ocean University,Zhoushan 316021,China;Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,Fishery Environment and Aquatic Products Quality Supervision and Testing Center (Guangzhou),Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China)

机构地区：[1]浙江海洋大学,浙江舟山316021 [2]中国水产科学研究院南海水产研究所农业农村部水产品加工重点实验室农业农村部渔业环境及水产品质量监督检验测试中心(广州),广东广州510300

出　　处：《南方水产科学》2019年第2期58-63,共6页South China Fisheries Science

基　　金：中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2017YB14;2017YB05;2016TS15);国家农产品质量安全风险评估重大专项(GJFP201600902)

摘　　要：水产麻醉剂丁香酚具有良好的抑菌和杀菌作用,不仅对食源性致病菌具有良好的抑制作用,也对水产致病菌具有潜在的抑菌效力。为探讨丁香酚对水产养殖业主要致病菌的抑菌作用,文章初步开展了丁香酚对鳗弧菌(Vibrio anguillarum)的抑菌效果研究。丁香酚质量浓度为6 400μg·mL^(-1)时,鳗弧菌培养平板中抑菌圈直径为(21.13±0.74) mm,表明鳗弧菌对此浓度的丁香酚表现为极敏。吸光度测定结果显示微孔中丁香酚质量浓度≥400μg·mL^(-1)时弧菌没有生长,表明丁香酚对鳗弧菌的最低抑菌浓度(MIC)为400μg·mL^(-1)。取400μg·mL^(-1)、800μg·mL^(-1)和1 600μg·mL^(-1)微孔中溶液涂抹于培养平板继续培养24 h,质量浓度≥800μg·mL^(-1)时鳗弧菌没有生长,表明最小杀菌浓度(MBC)为800μg·mL^(-1)。研究结果表明丁香酚对鳗弧菌具有良好的抑菌作用。Fish anesthetic eugenol,which has good antibacterial and bactericidal effects,has presented great efficacy on the food borne pathogens as well as the pathogenic bacteria in aquaculture.In order to investigate the antibacterial effect of eugenol on the typical pathogenic bacteria in aquaculture,we studied the antibacterial effect of eugenol on Vibrio anguillarum.After 24-hour incubation,the diameter of the inhibition zone was(21.13±0.74)mm at 6400 μg·mL^–1 of eugenol,indicating that V.anguillarum was extremely sensitive to eugenol.It is shown that V.anguillarum did not grow while the eugenol concentration was over 400 μg·mL^–1.Hence,the minimum inhibitory concentration(MIC)was 400 μg·mL^–1 of eugenol against the bacteria.The solution in the cells of 400 μg·mL^–1,800 μg·mL^–1 and 1 600 μg·mL^–1 were continually incubated for 24 h after being scribbled on the plate surface.When the concentration of eugenol were over 800 μg·mL^–1,no V.anguillarum grew in the plates.It is indicated that the minimum bactericidal concentration(MBC)of eugenol against V.anguillarum was 800 μg·mL^–1,and eugenol has excellent antibacterial effects on V.anguillarum.

关 键 词：丁香酚 鳗弧菌 抑菌圈 最低抑菌浓度 最小杀菌浓度 渔用麻醉剂 

分 类 号：S942.2[农业科学—水产养殖]