猛虾蛄卵巢发育特征及其纳精囊内容物超显微观察  

Developmental features of ovary and ultramicroscopic observation of thelycum contents of Harpiosquilla harpax

作　　者：赵旺[1,2] 杨其彬 陈旭[1,2] 陈明强 温为庚[1,2] ZHAO Wang;YANG Qibin;CHEN Xu;CHEN Mingqiang;WEN Weigeng(Tropical Aquaculture Research and Development Center,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Sanya 572000,China;Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China)

机构地区：[1]中国水产科学研究院南海水产研究所热带水产研究开发中心,海南三亚572000 [2]农业农村部南海渔业资源开发利用重点实验室,广东广州510300

出　　处：《南方水产科学》2019年第2期121-126,共6页South China Fisheries Science

基　　金：三亚市农业科技创新项目(2017NK01);海南省重点项目(ZDYF2018096)

摘　　要：该研究收集了168尾雌性猛虾蛄(Harpiosquilla harpax)并逐一解剖,比较了不同个体猛虾蛄卵巢和纳精结构的发育特征,对乳白色纳精结构内容物进行了超显微观察。结果显示,猛虾蛄具成对的卵巢,位于围心窦和消化道之间,从头胸甲后部延伸至尾节。卵巢发育颜色变化为无色-灰黄色-土黄色-浅黄色-橘黄色,成熟的卵巢呈橘黄色或粉红色。性腺指数分别为1.62±0.53、2.73±0.44、3.82±0.48、6.84±1.04和11.63±2.11;雌性虾蛄第6、第7、第8胸节腹部有3条平行带,为储存精子的器官,其表观颜色也有变化(无色透明-浅白色浑浊-乳白色),性腺成熟度越高,纳精结构乳白色越明显。其内容物具集群状态的精子,群外被包膜,精子间充满丝状间质,精子近球形,无鞭毛,无顶体,大小为1～3μm。研究结果可为猛虾蛄的繁殖生物学和人工育苗研究提供参考依据。We collected 168 female Harpiosquilla harpax and dissected them individually for a comparison of their developmental features of thelycum and ovary,and for ultramicroscopic observation of contents in the milky-white thelycum.The results show that the females had paired ovaries that lied between pericardial sinus and digestive tract,which extended from the back of the breastplate to the tail.The apparent color of ovary changed as follows:colorless.rayish yellow.earthy yellow.light yellow.orange,and the mature ovaries were orange or pink.The gonadosomatic index(GSI)were 1.62±0.53,2.73±0.44,3.82±0.48,6.84±1.04 and 11.63±2.11,respectively.The females had three parallel strips that were visible through the exoskeleton at the sixth,seventh,and eighth thoracic sternites,which were organs for sperm-storage,and the apparent colours of thelycum were changeable(colorless-transparent-light white-turbidity-milk white-dense).The more mature the ovary was,the heavier milk white of the thelycum became.Group of sperms with membranes had been observed in the thelycum contents which were filled with filamentous stroma.The shape of sperm was spherical but without flagellum and acrosome,size of 1~3 μm.The results provide references for further research on the reproductive biology and artificial breeding of H.harpax.

关 键 词：猛虾蛄 卵巢 纳精囊 精子 超显微观察 

分 类 号：S968[农业科学—水产养殖]