艾拉莫德治疗大鼠诱导性关节炎的机理研究  

Mechanisms of Elamode in treatment of induced arthritis in rats

作　　者：高鹏[1] 吴延龙 崔轶霞[1] Gao Peng;Wu Yanlong;Cui Yixia(Department of Immunology and Rheumatism,Yan'an University Affiliated Hospital,Yan'an,Shaanxi,716000,China)

机构地区：[1]延安大学附属医院免疫风湿科,陕西延安716000

出　　处：《西南国防医药》2019年第4期422-425,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的探究艾拉莫德(T-614)治疗大鼠诱导性关节炎(CIA)的机理。方法选取60只6周龄清洁级雌性大鼠,其中40只用于建立CIA模型,随机均分为模型对照组与艾拉莫德组;其余20只为空白对照组。模型对照组及空白对照组使用生理盐水灌胃,艾拉莫德组使用艾拉莫德灌胃治疗,干预时间均为4 w。干预结束后,使用酶联免疫吸附法(ELISA)测量各组血清白细胞介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)和B细胞活化因子(IL-6、IL-21)水平及脾脏淋巴细胞中B细胞总数,以及模型对照组与艾拉莫德组干预前后大鼠诱导关节肿胀率。结果 (1)干预前,模型对照组及艾拉莫德组血清IL-1、TNF-α、IL-6及IL-21水平明显高于空白对照组(P <0.05);干预后IL-1、TNF-α、IL-6及IL-21水平,模型对照组>艾拉莫德组>空白对照组(P <0.05)。(2)干预前,艾拉莫德组及模型对照组总B细胞数量高于空白对照组(P <0.05);干预后,艾拉莫德组总B细胞数量下降,且低于模型对照组(P <0.05)。(3)干预后,艾拉莫德组关节肿胀率低于模型对照组(P <0.05)。结论艾拉莫德能够显著改善诱导性关节炎大鼠关节肿胀症状,其机理与降低炎性因子水平和B细胞分泌功能有关。Objective To explore the mechanism of Elamode(T-614)in the treatment of collagen induced arthritis(CIA)in rats.Methods Sixty 6-week-old female rats of clean grade were selected,of which 40 ones were used to establish CIA model and they were randomly divided into a model control group and an Elamode group.The remaining 20 ones constituted a blank control group.The model control group and blank control group were given saline by gastric lavage,while the Elamode group was given Elamode by gastric lavage for four weeks.After the intervention,the levels of serum interleukin-1(IL-1),tumor necrosis factor-α(TNF-α),B cell activating factor(IL-6 and IL-21)and the total number of B cells in splenic lymphocytes,and the rate of joint swelling induced by the model control group and the Elamode group before and after the intervention were measured by use of ELISA.Results(1)Before the intervention,the serum levels of IL-1,TNFvα,IL-6 and IL-21 in the model control group and Elamode group were significantly higher than those in the blank control group(P<0.05);after the intervention,the sequence of levels of IL-1,TNF-α,IL-6 and IL-21 in the groups was:model control group>Elamode group>blank control group(P<0.05).(2)Before the intervention,the total number of B cells in the Elamode group and model control group was higher than that in the blank control group(P<0.05);after the intervention,the total number of B cells in the Elamode group decreased and was lower than that in the model control group(P<0.05).(3)After the intervention,the rate of joint swelling in the Elamode group was lower than that in the model control group(P<0.05).Conclusion Elamode can significantly improve the symptoms of joint swelling in rats with induced arthritis,and its mechanism is related to reducing the level of inflammatory factors and the secretory function of B cells.

关 键 词：艾拉莫德 大鼠 关节炎 B细胞 分泌功能 

分 类 号：R593.22[医药卫生—内科学]