高效液相色谱法测定乳酸依沙吖啶凝胶中乳酸依沙吖啶的含量  

Content Determination of Ethacridine Lactate in Ethacridine Lactate Gel by HPLC

作　　者：王风华 张献冲 WANG Feng-hua;ZHANG Xian-chong(Hunan Prevention and Treatment Center for Occupational Diseases,Changsha 410007,China;KANGDUOBAO Medical Health Products(Hunan)CO.,LTD,Changsha 410100,China)

机构地区：[1]湖南省职业病防治院,长沙410007 [2]康多宝医疗健康产品(湖南)有限公司,长沙410100

出　　处：《海峡药学》2019年第3期56-57,共2页Strait Pharmaceutical Journal

摘　　要：目的应用高效液相色谱法测定乳酸依沙吖啶凝胶中乳酸依沙吖啶的含量。方法色谱柱为Agilent TC-C_(18)(2)(4.6mm×150mm,5μm),以含0.1%辛烷磺酸钠的溶液[磷酸盐缓冲液(取磷酸二氢钠7.8g,加水900mL溶解后,用磷酸调节pH值至2.8,用水稀释至1000mL,即得)]-乙腈(700∶300)为流动相,流速1.0mL·min^(-1),柱温30℃,检测波长270nm,进样量:20μL。结果乳酸依沙吖啶在49.05～588.60ng范围内线性关系良好(r=0.9998),平均回收率为99.4%(RSD=1.9%,n=5)。结论该方法准确、简便、快速,可用于乳酸依沙吖啶凝胶的质量控制。OBJECTIVE To establish an HPLC method for the assay of ethacridine lactate in ethacridine lactate gel. METHODS In this system of HPLC,an Agilent TC-C18( 2) column( 4. 6 mm × 150 mm,5μm) was used as analytical column. Using 0. 1% Sodium octanesulfonate solution[Phosphate buffer( add 7. 8 g sodium dihydrogen phosphate into 900 mL water,adjusted to pH 2. 5 with phosphoric acid,then add water to 1000 mL ]-acetonitrile( 700∶300) as the mobile phase,the flow rate was 1. 0 mL·min^-1 and the column temperature was maintained at30℃ with the detective wavelength was set at 270 nm,The inject volume was 20μL. RESULTS The linear range was 49. 05 ~ 588. 60 ng,r = 0. 9998. The average recovery was 99. 8%,and RSD = 2. 0%( n = 5). CONCLUSION The method is convenient,rapid,accurate,and suitable for the determination of ethacridine lactate gel.

关 键 词：乳酸依沙吖啶 凝胶 HPLC 

分 类 号：R917[医药卫生—药物分析学]