分子标记辅助选择Pigm基因改良湘晚籼13号的稻瘟病抗性  

Improving the blast resistance of Xiangwanxian 13 by molecular marker-assisted selection of the Pigm gene

作　　者：赖怡帆 孙君玥 张旭辉 梁毅[1] 李永聪[1] 廖花[1] 黄俊[1] 吴婷婷 刘雄伦[1,2,3,4] LAI Yifan;SUN Junyue;ZHANG Xuhui;LIANG Yi;LI Yongcong;LIAO Hua;HUANG Jun;WU Tingting;LIU Xionglun(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Rice and Rapeseed Disease Resistance Breeding Key Laboratory of Hunan Province,Changsha,Hunan 410128,China;Crop Gene Engineering Key Laboratory of Hunan Province,Changsha,Hunan 410128,China;Southern Regional Collaborative Innovation Center for Grain and Oil Crops,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]水稻油菜抗病育种湖南省重点实验室,湖南长沙410128 [3]作物基因工程湖南省重点实验室,湖南长沙410128 [4]南方粮油作物协同创新中心,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2019年第2期113-117,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家重大专项(2016ZX08001–002);国家重点研发计划项目(2016YFD0101103–2);湖南省科技计划项目(2017NK2011)

摘　　要：以湘晚籼13号、谷梅4号、75–1–127(CK1)和CO39(CK2)为材料,用21份来自不同稻区的稻瘟菌菌株室内接种,采用室内苗瘟鉴定和田间病圃鉴定的方法,明确Pigm基因供体亲本谷梅4号和受体亲本湘晚籼13号的稻瘟病抗性表现及抗菌谱;根据Pigm基因序列信息,设计和筛选高效多态分子标记T9E4;利用获选分子标记,开展分子标记辅助选择(MAS)连续回交育种实践,培育高抗病株系。结果表明:Pigm基因供体亲本谷梅4号和受体亲本湘晚籼13号的抗性反应及抗菌谱存在明显差异,抗性频率分别为95.2%和61.9%。大田病圃稻瘟病抗性鉴定表明,谷梅4号高抗苗瘟和穗瘟,而湘晚籼13号高感苗瘟和穗瘟。T9E4标记引物在谷梅4号和湘晚籼13号基因组中分别扩增出1条约750 bp和1条约1 800 bp的稳定明亮条带,且PCR产物可以用琼脂糖凝胶电泳快速检测,标记辅助选择效率达100%。通过连续回交、自交及分子选择,培育出1个含有Pigm纯合等位基因、高抗苗瘟和穗瘟,且主要农艺性状与湘晚籼13号高度相似的BC5F3株系,实现了定向改良目标。A PCR-based co-dominant InDel marker, T9E4, was developed according to sequence information of the broad-spectrum and durable blast resistance gene Pigm, and was employed in molecular marker-assisted selection breeding for blast resistance improvement of the rice variety Xiangwanxian 13. Resistance responses to 21 tested Magnaporthe oryzae isolates were quite different between the Pigm donor Gumei 4 and receptor Xiangwanxian 13, presenting 95.2% and 61.9% resistance frequency respectively in Greenhouse inoculation assay. Gumei 4 showed high-level seedling and panicle blast resistance but Xiangwanxian 13 was highly susceptible in field nursery. The T9E4 marker primers amplified a nearly 750 bp band from Gumei 4 genome while the PCR product is about 1 800 bp from the receptor DNA template, which can be conveniently detected by agarose gel electrophoresis, and the marker-assisted selection efficiency reached 100% in this study. An elite BC 5 F 3 line, harboring Pigm homozygous allele, possessing high-level resistance to seedling and panicle blast, and with similar agronomic traits to the receptor, was bred byconsecutive backcross, self-fertilization and molecular selection.

关 键 词：水稻 稻瘟病 抗性改良 分子标记辅助选择 Pigm基因 

分 类 号：S511.035.3[农业科学—作物学]